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Development of a High-Sensitivity Quantitation Method for Arginine Vasopressin by High-Performance Liquid Chromatography Tandem Mass Spectrometry, and Comparison with Quantitative Values by Radioimmunoassay

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Abstract

Human plasma arginine vasopressin (AVP) levels serve as a clinically relevant marker of diabetes and related syndromes. We developed a highly sensitive method for measuring human plasma AVP using high-performance liquid chromatography tandem mass spectrometry. AVP was extracted from human plasma using a weak-cation solid-phase extraction plate, and separated on a wide-bore octadecyl reverse-phase column. AVP was quantified in ion-transition experiments utilizing a product ion (m/z 328.3) derived from its parent ion (m/z 542.8). The sensitivity was enhanced using 0.02% dichloromethane as a mobile-phase additive. The lower limit of quantitation was 0.200 pmol/L. The extraction recovery ranged from 70.2 ± 7.2 to 73.3 ± 6.2% (mean ± SD), and the matrix effect ranged from 1.1–1.9%. Quality-testing samples revealed interday/intraday accuracy and precision ranging over 0.9–3% and–0.3–2%, respectively, which included the endogenous baseline. Our results correlated well with radioimmunoassay results using 22 human volunteer plasma samples.

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Authors and Affiliations

  1. Analytical Technology Research Department, LSI Medience Corp., 2-1-6 Sengen, Tsukuba, Ibaraki, 305-0047, Japan

    Yasuko Tsukazaki & Naoto Senda

  2. Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan

    Naoto Senda, Kinya Kubo, Hiroyuki Kugoh, Yasuhiro Kazuki & Mitsuo Oshimura

  3. K. K. AB SCIEX, 4-7-35 Kitashinagawa, Shinagawa, Tokyo, 140-0001, Japan

    Shigeru Yamada

  4. Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan

    Hiroyuki Kugoh & Yasuhiro Kazuki

Authors
  1. Yasuko Tsukazaki
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  2. Naoto Senda
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  3. Kinya Kubo
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  4. Shigeru Yamada
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  5. Hiroyuki Kugoh
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  6. Yasuhiro Kazuki
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  7. Mitsuo Oshimura
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Correspondence to Yasuko Tsukazaki.

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Tsukazaki, Y., Senda, N., Kubo, K. et al. Development of a High-Sensitivity Quantitation Method for Arginine Vasopressin by High-Performance Liquid Chromatography Tandem Mass Spectrometry, and Comparison with Quantitative Values by Radioimmunoassay. ANAL. SCI. 32, 153–159 (2016). https://doi.org/10.2116/analsci.32.153

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  • DOI: https://doi.org/10.2116/analsci.32.153

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