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Colorimetric Determination of Sulfide in Microsamples

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Abstract

A method for determination of hydrogen sulfide in microsamples (200 μL) was developed by modifying the methylene blue method. Samples were collected using a micropipette and were combined with sulfide coloring reagent and 5 mL of 0.1 M HCl in test tubes. Absorbance of the solution was measured spectrophotometrically at 667 nm. This modified method did not require any special labware or technique, and can be used in a variety of research fields.

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Acknowledgments

Funding was provided by the River Technology Research and Development System, Regional Issues Field (River Ecosystems) and by consignment study from Shimane Prefecture.

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Authors and Affiliations

  1. Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu, Matsue, Shimane, 690-8504, Japan

    Shogo Sugahara, Mai Suzuki, Michiko Egawa & Yasushi Seike

  2. Shimane Prefectural Institute of Public Health and Environmental Science, 582-1 Nishihamasada, Matsue, Shimane, 690-0122, Japan

    Hiroshi Kamiya

  3. Institute of Geology and Geoinformation, Geological Survey of Japan (GSJ), AIST, Central 7, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8567, Japan

    Masumi Yamamuro

  4. Inland Water Fisheries and Coastal Fisheries Division, Shimane Prefectural Fisheries Technology Center, 1659-1 Okinoshima, Sono-cho, Izumo, Shimane, 691-0076, Japan

    Hitoshi Semura

  5. Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba, 274-8510, Japan

    Yukiko Senga

Authors
  1. Shogo Sugahara
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  2. Mai Suzuki
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  3. Hiroshi Kamiya
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  4. Masumi Yamamuro
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  5. Hitoshi Semura
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  6. Yukiko Senga
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  7. Michiko Egawa
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  8. Yasushi Seike
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Corresponding author

Correspondence to Shogo Sugahara.

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Sugahara, S., Suzuki, M., Kamiya, H. et al. Colorimetric Determination of Sulfide in Microsamples. ANAL. SCI. 32, 1129–1131 (2016). https://doi.org/10.2116/analsci.32.1129

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  • DOI: https://doi.org/10.2116/analsci.32.1129

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