Abstract
Glucose dehydrogenase (GlDH) and ferrocene were coadsorbed on a carbon felt (CF) sheet (5 × 10 mm, 2 mm thickness), which was used to construct an electrode for the electrochemical detection of glucose. A potential of +0.3 V vs. Ag/AgCl was applied on the base CF, and the current was measured. After the addition of glucose, the current increased and reached a steady state within 50 s. The current response was proportional to the glucose concentration up to 20 μM, with a lower detection limit of 1 μM. The surface of the CF electrode was covered by layers of polystyrene sulfonate and poly-L-lysine using layer-by-layer technique. Again the current response was proportional to glucose concentration up to 20 μM, with a lower detection limit of 2 μM. The oxidation current owing to electrochemical interferents such as L-ascorbate and acetaminophen was 1/8 times of the current observed on the unprotected electrode. In addition, the protection imparted stability to the electrode. Our work demonstrates that a GlDH/ferrocene CF electrode, protected with polystyrene sulfonate and poly-L-lysine, could be used for the electrochemical detection of glucose.
Similar content being viewed by others
References
P. Leung, X. Li, P. de León, L. Berlouis, C.T.J. Low, F.C. Walsh, RSCAdv., 2012, 2, 10125.
A.I. Tsyganok, Res. Chem. Intermed., 2006, 32, 357.
S. Uchiyama, S. Kato, S. Suzuki, O. Hamamoto, Electroanalysis, 1991, 3, 59.
S. Uchiyama and S. Suzuki, Sens. Actuators, B, 1993, 13, 76.
F. Mizutani and S. Yabuki, Chem. Lett., 1994, 1569.
F. Mizutani and S. Yabuki, Sens. Actuators, B, 1994, 25, 750.
S. Uchiyama, Y. Hasebe, M. Tanaka, Electroanalysis, 1997, 9, 176.
S. Tsujimura, B. Tatsumi, J. Ogawa, S. Shimizu, K. Kano, T. Ikeda, J. Electroanal. Chem., 2001, 496, 69.
S. Uchiyama, M. Kobayashi, Y. Hasebe, Electroanalysis, 2002, 14, 1644.
Y. Wang and Y. Hasebe, Talanta, 2009, 79, 1135.
Y. Wang and Y. Hasebe, Anal. Bioanal. Chem., 2011, 399, 1151.
J. Shim, G.-Y. Kim, S.-H. Moon, J. Electroanal. Chem., 2011, 653, 14.
Y. Wang and Y. Hasebe, J. Pharm. Biomed. Anal., 2012, 57, 125.
Y. Handa, K. Yamagiwa, Y. Ikeda, Y. Yanagisawa, S. Watanabe, N. Yabuuchi, S. Komaba, ChemPhysChem, 2014, 15, 2145.
S. Yabuki and F. Mizutani, Biosens. Bioelectron., 1995, 10, 353.
F. Mizutani, S. Yabuki, Y. Hirata, Anal. Chim. Acta, 1995, 314, 233.
F. Mizutani, S. Yabuki, Y. Hirata, Talanta, 1996, 43, 1815.
F. Mizutani, Y. Sato, Y. Hirata, T. Sawaguchi, S. Yabuki, Anal. Chim. Acta, 1998, 364, 173.
S. Yabuki, F. Mizutani, Y. Hirata, J. Electroanal. Chem., 1999, 468, 117.
S. Yabuki, F. Mizutani, Y. Hirata, Electroanalysis, 2001, 13, 380.
S. Yabuki, S. Fujii, F. Mizutani, Y. Hirata, Anal. Biochem., 2008, 375, 141.
S. Yabuki, Anal. Sci., 2011, 27, 695.
T. Hoshi, H. Saiki, S. Kuwazawa, C. Tsuchiya, Q. Chen, J. Anzai, Anal. Chem., 2001, 73, 5310.
A.H. Liu and J. Anzai, Langmuir, 2003, 19, 4043.
T. Hoshi, H. Saiki, J. Anzai, Talanta, 2003, 61, 363.
H.B. Shi, Z. Song, J.D. Huang, Y. Yang, Z.X. Zhao, J. Anzai, T. Osa, Q. Chen, Sens. Actuators, B, 2005, 109, 341.
T. Hoshi, N. Sagae, K. Daikuhara, J. Anzai, Talanta, 2007, 71, 644.
P. Scodeller, R. Carballo, R. Szamocki, L. Levin, F. Forchiassin, E.J. Calvo, J. Am. Chem. Soc., 2010, 132, 11132.
R. Perazzini, R. Saladino, M. Guazzaroni, C. Crestini, Bioorg. Med. Chem., 2011, 19, 440.
S.M. Tangutooru, V.L. Kopparthy, G.G. Nestorova, E.J. Guilbeau, Sens. Actuators, B, 2012, 166, 637.
K. Sato, S. Takahashi, J. Anzai, Anal. Sci., 2012, 28, 929.
J.S. Graca, R.F. de Oliveira, M.L. de Moraes, M. Ferreira, Bioelectrochemistry, 2014, 96, 37.
S. Yabuki, Anal. Sci., 2014, 30, 213.
Datasheet of Glucose Dehydrogenase (PQQ-dependent) (GLD-321), Toyobo, Osaka, http://www.toyobo-global.com/seihin/xr/enzyme/pdf_files/2010_105_108_GLD_321.pdf.
S. Yabuki and S. Fujii, Microchim. Acta, 2009, 164, 173.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yabuki, S., Hirata, Y. Enzyme and Mediator-coadsorbed Carbon Felt Electrode for Electrochemical Detection of Glucose Covered with Polymer Layers Based on Layer-by-Layer Technique. ANAL. SCI. 31, 693–697 (2015). https://doi.org/10.2116/analsci.31.693
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.31.693