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Affinity Capillary Electrophoresis for Selective Control of Electrophoretic Mobility of Sialic Acid Using Lanthanide-Hexadentate Macrocyclic Polyazacarboxylate Complexes

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Abstract

It is difficult to control the electrophoretic mobility in order to obtain high resolution among saccharides in complex samples. We report herein on a new affinity capillary electrophoresis (ACE) method for an anionic monosaccharide, N-acetylneuraminic acid (Neu5Ac), which is important in terms of pathological diagnosis, using lanthanide-hexadentate macrocyclic polyazacarboxylate complexes (Ln-NOTA) as affinity reagents. It was shown that Ln-NOTA complexes increased the anionic mobility of Neu5Ac by approximately 40% through selective complexation with Neu5Ac. The extent of change in the mobility strongly depended on the type of central metal ion of Ln-NOTA. The stability constant (K) of Lu-NOTA with Neu5Ac was determined by ACE to be log Kb = 3.62 ± 0.04, which is the highest value among artificial receptors for Neu5Ac reported so far. Using this ACE, the Neu5Ac content in a glycoprotein sample, α1-acid glycoprotein (AGP), was determined after acid hydrolysis. Complete separation between Neu5Ac and hydrolysis products was successful by controlling the mobility to determine the concentration of Neu5Ac.

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Authors and Affiliations

  1. Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, 338-8570, Sakura, Saitama, Saitama, Japan

    Daiki Goto, Kazuki Ouchi, Masami Shibukawa & Shingo Saito

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  1. Daiki Goto
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  2. Kazuki Ouchi
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  3. Masami Shibukawa
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  4. Shingo Saito
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Correspondence to Shingo Saito.

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Goto, D., Ouchi, K., Shibukawa, M. et al. Affinity Capillary Electrophoresis for Selective Control of Electrophoretic Mobility of Sialic Acid Using Lanthanide-Hexadentate Macrocyclic Polyazacarboxylate Complexes. ANAL. SCI. 31, 1143–1149 (2015). https://doi.org/10.2116/analsci.31.1143

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  • DOI: https://doi.org/10.2116/analsci.31.1143

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