Abstract
Conformational changes of hydrated proteins induced by gradual dehydration were monitored by vibrational circular dichroism (VCD) spectroscopy. In myoglobin and casein, representative α-helix-rich and random-coil proteins, respectively, an increase in left-handed optical activity in the amide I band was detected at the initial stage of dehydration, followed by an increase in opposite right-handed activity in both the amide I and II bands with further dehydration. Because the second step was observed with an increase in the turbidity of the proteins, it can be attributed to their aggregation. In contrast, because the increase in left-handed optical activity is induced by the conformational change of the proteins and is followed by the aggregation, it may derive from the increase in the regularity of the local structure in individual myoglobin or casein that triggers the aggregation.
Similar content being viewed by others
References
L. M. Mannuzzu, M. M. Moronne, and E. Y. Isacoff, Science, 1996, 271, 213.
S. Yamamoto, G. P. Subedi, S. Hanashima, T. Satoh, M. Otaka, H. Wakui, K. Sawada, S. Yokota, Y. Yamaguchi, H. Kubota, and H. Itoh, J. Biol. Chem., 2014, 289, 9880.
T. Oka, K. Inoue, M. Kataoka, and N. Yagi, Biophys. J., 2005, 88, 436.
Y. Nakasone, K. Zikihara, S. Tokutomi, and M. Terazima, Biophys. J., 2010, 99, 3831.
C. V. Pagba and B. A. Barry, J. Phys. Chem. B, 2012, 116, 10590.
X. Ding, X. Zhao, and A. Watts, Biochem. J., 2013, 450, 443.
F. Korkmaz, S. Ressl, C. Ziegler, and W. Mäntele, Biochim. Biophys. Acta, 2013, 1828, 1181.
K. Takeda, Y. Nakasone, K. Zikihara, S. Tokutomi, and M. Terazima, J. Phys. Chem. B, 2013, 117, 15606.
C. M. Dobson, Nature, 2003, 426, 884.
N. Gregersen, P. Bross, S. Vang, and J. H. Christensen, Annu. Rev. Genomics Hum. Genet., 2006, 7, 103.
F. Chiti and C. M. Dobson, Nat. Chem. Biol., 2009, 5, 15.
J. J. Yerbury, E. M. Stewart, A. R. Wyatt, and M. R. Wilson, EMBO Rep., 2005, 6, 1131.
F. Meersman, L. Smeller, and K. Heremans, Biophys. J., 2002, 82, 2635.
G. Shanmugam and P. L. Polavalapu, Biophys. Chem., 2004, 111, 73.
A. Hédoux, Y. Guinet, and L. Paccou, J. Phys. Chem. B, 2011, 115, 6740.
S. Yamamoto and H. Watarai, Chirality, 2012, 24, 97.
Q. Wang, Y. Wang, and H. P. Lu, J. Raman Spectrosc., 2013, 44, 670.
A. E. Mark and W. F. van Gunsteren, Biochemistry, 1992, 31, 7745.
A. Caflisch and M. Karplus, J. Mol. Biol., 1995, 252, 672.
S. Chowdhury, H. Lei, and Y. Duan, J. Phys. Chem. B, 2005, 109, 9073.
(a) S. J. Prestrelski, N. Tedeschi, T. Arakawa, and J. F. Carpenter, Biophys. J., 1993, 65, 661. (b) A. M. Squires, G. L. Devlin, S. L. Gras, A. K. Tickler, C. E. MacPhee, and C. M. Dobson, J. Am. Chem. Soc., 2006, 128, 11738. (c) F. Mallamace, S.-H. Chen, M. Broccio, C. Corsaro, V. Crupi, P. Baglioni, E. Fratini, C. Vannucci, and H. E. Stanley, J. Chem. Phys., 2007, 127, 045104. (d) S. Mukherjee, P. Chowdhury, and F. Gai, J. Phys. Chem. B, 2007, 111, 4596. (e) J. Zhang and Y. B. Yan, Protein Pept. Lett., 2008, 15, 650. (f) A. Hédoux, L. Paccou, S. Achir, and Y. Guinet, J. Pharm. Sci., 2012, 101, 2316. (g) M Adrover, G. Martorell, S. R. Martin, D. Urosev, P. V. Konarev, D. I. Svergun, X. Daura, P. Temussi, and A. Pastore, J. Mol. Biol., 2012, 417, 413.
(a) N. Sengupta, S. Jaud, and D. J. Tobias, Biophys. J., 2008, 95, 5257. (b) S. Chankraborty and S. Bandyopadhyay, J. Phys. Chem. B, 2008, 112, 6500. (c) Y. Zhang, M. Lagi, D. Liu, F. Mallamace, E. Fratini, P. Baglioni, E. Mamontov, M. Hagen, and S.-H. Chen, J. Chem. Phys., 2009, 130, 135101. (d) S. Abel, M. Waks, and M. Marchi, Eur. Phys. J. E, 2010, 32, 399. (e) P. Das and S. Matysiak, J. Phys. Chem. B, 2012, 116, 5342.
P. Pancoska, S. C. Yasui, and T. A. Keidering, Biochemistry, 1989, 28, 5917.
V. P. Gupta and T. A. Keiderling, Biopolymers, 1992, 32, 239.
G. Shanmugam and P. L. Polavalapu, J. Am. Chem. Soc., 2004, 126, 10292.
S. Ma, X. Cao, M. Mak, A. Sadik, C. Walkner, T. B. Freedman, I. K. Lednev, R. K. Dukor, and L. A. Nafie, J. Am. Chem. Soc., 2007, 129, 12364.
T. J. Measey, K. B. Smith, S. M. Decatur, L. Zhao, G. Yang, and R. Schweitzer-Stenner, J. Am. Chem. Soc., 2009, 131, 18218.
T. J. Measey and R. Schweitzer-Stenner, J. Am. Chem. Soc., 2011, 133, 1066.
D. Kurouski, R. K. Dukor, X. Lu, L. A. Nafie, and I. K. Lednev, Biophys. J., 2012, 103, 522.
T. Iwata, A. Yamamoto, S. Tokutomi, and H. Kandori, Biochemistry, 2007, 46, 7016.
S. Ye and A. Markelz, J. Phys. Chem. B, 2010, 114, 15151.
J. Vojtěchovský, K. Chu, J. Berendzen, R. M. Sweet, and I. Schlichting, Biophys. J., 1999, 77, 2153.
M. Darewicz and J. Dziuba, Eur. Food Res. Technol., 2007, 226, 147.
K. Konno, I. Shiina, and H. Yui, J. Mol. Struct., 2013, 1035, 260.
D. Lin-Vien, N. B. Colthup, W. G. Fateley, and J. G. Grasselli, “The Handbook of Infrared and Raman Characteristic Frequencies of Organic Molecules”, 1991, Academic Press, Boston.
M. D. Shoulders and R. T. Raines, Annu. Rev. Biochem., 2009, 78, 929.
J. P. R. O. Orgel, T. C. Irving, A. Miller, and T. J. Wess, Proc. Natl. Acad. Sci. U. S. A., 2006, 103, 9001.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Morisaku, T., Arai, S. & Yui, H. Dehydration-induced Initial Conformational Change of Hydrated Proteins Detected by the Changes in Vibrational Circular Dichroism Activity. ANAL. SCI. 30, 961–969 (2014). https://doi.org/10.2116/analsci.30.961
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.30.961