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A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose

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Abstract

We developed a novel metal adsorbent composed of bio-based materials, cellulose and a protein. The approach involved the immobilization of a hexa-histidine tag (His6), which shows an affinity for an intermediate acid (metal ion) in Hard and Soft Acids and Bases (HSAB) theory, on cellulose by fusing with a carbohydrate-binding module (CBM). The results show that CBM-His6-bound cellulose has adsorption selectivity reflecting the original properties of His6. Additionally, we prepared three configurations of CBM-His6 proteins, which were subsequently immobilized on filter paper for Ni2+ ion adsorption. Of these configurations, we found that the protein containing two His6 tags at each terminus (N- and C-) of CBM exhibited the highest metal adsorption ability. Furthermore, XPS analysis confirmed the binding of Ni2+ ions on the cellulose.

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Acknowledgments

This work was partly supported by JSPS KAKENHI grant numbers JP18H03395 and JST/JICA, SATREPS (Science and Technology Research Partnership for Sustainable Development: JPMJSA1501). A part of this work was conducted at the Laboratory of XPS analysis, Hokkaido University, supported by “Nanotechnology Platform” Program of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

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Authors and Affiliations

  1. Division of Sustainable Resources Engineering, Faculty of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita, Sapporo, 060-8628, Japan

    Yuki Togo, Kazunori Nakashima, Wilson Mwandira & Satoru Kawasaki

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  1. Yuki Togo
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  2. Kazunori Nakashima
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  3. Wilson Mwandira
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  4. Satoru Kawasaki
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Correspondence to Kazunori Nakashima.

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Togo, Y., Nakashima, K., Mwandira, W. et al. A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose. ANAL. SCI. 36, 459–464 (2020). https://doi.org/10.2116/analsci.19P356

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  • DOI: https://doi.org/10.2116/analsci.19P356

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