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An Ultrasensitive Colorimetric Strategy for Detection of Cadmium Based on the Peroxidase-like Activity of G-Quadruplex-Cd(II) Specific Aptamer

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Abstract

We rationally designed an ultrasensitive and label-free sensing platform for determination of cadmium (Cd). The sensing platform contains G-quadruplex-Cd(II) specific aptamer (GCDSA) constructed by incorporating G-rich sequence at the end of 5' and the critical domain of the Cd-4 aptamer. GCDSA designed act as both a special recognition sequence for Cd2+ and a signal DNAzyme. In absence of Cd2+, GCDSA may mainly exist in a random coil sequence. Upon addition of Cd2+, GCDSA could probably be induced to fold into a G-quadruplex structure. The generation of plentiful active G-quadruplex interacts with hemin to form a peroxidase-like DNAzyme, leading to increased absorbance signal of the sensing system. A4 was directly proportional to the two segments of concentrations for Cd2+, with the detection of limit of 0.15 nM. The proposed method avoids the labeled oligonucleotides and allows directly quantitative analysis of the samples by cheap instruments, with an excellent dynamic range.

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Acknowledgments

This work is subsidized by the National Natural Science Foundation of China (No. 81502850), the Natural Science Foundation of Hunan Province in China (No. 2015JJ2122) and Undergraduate inquiry learning and innovative experimental project in Hunan province (No. 336).

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    Authors and Affiliations

    1. Wuhan Children’s Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, 100 Hong Kong Road, Jiang’an District, Wuhan City, Hubei Province, 430016, China

      Bin Zhou, Xi-Jiang Hu & Qing-Li Suo

    2. College of Public Health, University of South China, 28 Chang sheng West Road, Hengyang City, Hunan Province, 421001, China

      Ya-Ting Chen, Xin-Yi Yang & Yong-Sheng Wang

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    1. Bin Zhou
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    2. Ya-Ting Chen
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    5. Xi-Jiang Hu
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    Correspondence to Bin Zhou.

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    Zhou, B., Chen, YT., Yang, XY. et al. An Ultrasensitive Colorimetric Strategy for Detection of Cadmium Based on the Peroxidase-like Activity of G-Quadruplex-Cd(II) Specific Aptamer. ANAL. SCI. 35, 277–282 (2019). https://doi.org/10.2116/analsci.18P248

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