Abstract
ε-Poly-L-lysine (εPL) is a homopolymer of L-lysine residues with linkages between the α-carboxyl and ε-amino groups. εPL exists as a polycationic species under acidic and neutral conditions, and can form a polyion complex with negatively charged glucose oxidase (GOx). We previously reported that εPL significantly promoted a GOx enzymatic reaction using ferricyanide ion ([Fe(CN)6]3–) as the oxidant. Here, we construct a GOx-immobilized electrode using εPL and glutaraldehyde, and show that [Fe(CN)6]3– can efficiently act as the mediator in this electrode. Ferricyanide ion failed to function adequately as the mediator when bovine serum albumin or other polyamines (e.g., polyallylamine, α-poly-Llysine) were used instead of εPL. The GOx-immobilized electrode using εPL successfully responded to glucose even when the [Fe(CN)6]3– concentration was as low as 1 mM, and exhibited a wide dynamic range of up to several tens of mM. Thus, εPL is considered to be a useful additive for glucose sensors based on the [Fe(CN)6]4–/3–-mediated GOx catalytic current.
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Acknowledgments
This work was supported in part by a Japan Society for the Promotion of Science KAKENHI Grant (no. 17K14503).
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Uematsu, K., Ueno, T. & Katano, H. Effect of £-Poly-l-lysine on a Glucose Sensor Based on Glucose Oxidase and Ferricyanide Ion. ANAL. SCI. 34, 947–951 (2018). https://doi.org/10.2116/analsci.18P114
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DOI: https://doi.org/10.2116/analsci.18P114