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BIOCHIP mosaic for the diagnosis of autoimmune bullous diseases in Chinese patients

  • Investigative Report
  • Published:
European Journal of Dermatology

Abstract

Background

Autoimmune blistering diseases (AIBDs) are a group of fatal diseases with specific autoantibodies. BIOCHIP mosaic is a novel and all-in-one measure used for the rapid diagnosis of AIBDs.

Objectives

To evaluate the diagnostic accuracy based on BIOCHIP mosaic (FA1501-1005-60) in Chinese patients with AIBDs.

Materials and Methods

Seventy-seven patients with AIBDs and 20 controls were enrolled. The BIOCHIP mosaic was performed using both serum and plasma samples.

Results

Based on BIOCHIP mosaic, the data from paired plasma and serum samples demonstrated a high degree of concordance (Cohen’s kappa = 0.896–1.000) for autoantibodies against Dsgl, Dsg3, BP180-NC16A-4X, BP230gC, prickle-cell desmosomes, and pemphigoid antigens. Moreover, BIOCHIP mosaic also demonstrated a high degree of consistency for the detection rate of anti-Dsgl, Dsg3, plakins, BP180-NC16A-4X and non-collagenous domain of type VII collagen autoantibodies for the diagnosis of pemphigus foliaceus (77.3%), pemphigus vulgaris (88.6%), paraneoplastic pemphigus (100.0%), bullous pemphigoid (92.8%) and epidermolysis bullosa acquisita (99.0%), respectively.

Conclusion

Using BIOCHIP mosaic, serum and plasma samples may be used interchangeably at 1/10 dilution. Overall, the BIOCHIP mosaic was shown to be a useful and accurate tool for the diagnosis of AIBDs.

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Author notes

  1. These authors contributed equally

Authors and Affiliations

  1. Department of Dermatology, Peking University First Hospital, and National Clinical Research Center for Skin and Immune Diseases, and Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, China

    Mingyue Wang, Furong Li, Xue Wang, Rui Wang, Tiancheng Chen, Junyu Zhao, Xixue Chen & Xuejun Zhu

Authors
  1. Mingyue Wang
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  2. Furong Li
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  3. Xue Wang
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  4. Rui Wang
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  5. Tiancheng Chen
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  6. Junyu Zhao
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  7. Xixue Chen
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  8. Xuejun Zhu
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Corresponding authors

Correspondence to Xixue Chen or Xuejun Zhu.

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Disclosure

Acknowledgements: The BIOCHIP mosaic was obtained from a EUROIMMUN (Germany) project, entitled: “prospective multicentre study on serum autoantibodies and genetic susceptibility in autoimmune bullous diseases.” Funding sources: National Natural Science Foundation of China (grant number: 81130030 and 81000694). Conflicts of interest: none.

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Wang, M., Li, F., Wang, X. et al. BIOCHIP mosaic for the diagnosis of autoimmune bullous diseases in Chinese patients. Eur J Dermatol 30, 338–344 (2020). https://doi.org/10.1684/ejd.2020.3839

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  • DOI: https://doi.org/10.1684/ejd.2020.3839

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