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Fibroblast and keratinocyte crosstalk: the effect of a poly(tri[ethylene glycol] ethyl ether methacrylate) thermoresponsive surface on short-term co-culture

  • Investigative Report
  • Published:
European Journal of Dermatology Aims and scope

Abstract

Background

The treatment of difficult-to-treat wounds can be challenging. Although a number of approaches have been investigated, the healing process may be slow and unsatisfactory. An alternative approach is the use of a continuous sheet of skin cells applied over a wound which may improve cell implantation and patient recovery.

Objectives

To analyse the gene expression profile of fibroblast/keratinocyte co-culture on poly(tri[ethylene glycol] ethyl ether methacrylate) (P[TEGMA-EE]), a thermoresponsive biocompatible surface.

Materials and Methods

Cultures were grown for 72 hours as a continuous layer on P(TEGMA-EE). Assays for genotoxicity, cell morphology, and fluorescence-assisted flow cytometry were performed to exclude adverse effects. A gene expression profile related to the extracellular matrix was investigated by microarray analysis.

Results

for fibroblast monocultures and fibroblast/keratinocyte co-cultures maintained for 72 hours on P(TEGMA-EE), no change in morphology or specific surface markers, or DNA damage (comet assay) was observed, relative to control surface. Moreover, no detrimental impact was ascertained based on microarray analysis. In response to lowered temperature, the detachment of a continuous cell layer sheet from the thermoresponsive surface was observed. When gene expression was compared between fibroblasts cultured alone and co-cultured with keratinocytes on P(TEGMA-EE), 10 genes were shown to be differentially expressed. Of these genes, six were significantly differentially expressed between cultures grown on P(TEGMA-EE) and human skin samples.

Conclusion

Our results indicate that P(TEGMA-EE) is fully biocompatible and is therefore a suitable surface for successful preparation and recovery of two-layered fibroblast/keratinocyte co-culture as a continuous sheet of cells.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, ul. Medyków 18, Bldg. C-1, 40-752, Katowice, Poland

    Marta Lesiak, Łukasz Sieroń, Ewa Gutmajster & Aleksander L. Sieroń

  2. School of Medicine in Katowice, Medical University of Silesia in Katowice, Katowice, Poland

    Marta Lesiak, Łukasz Sieroń, Ewa Gutmajster & Aleksander L. Sieroń

  3. Centre of Polymer and Carbon Materials, Polish Academy of Sciences, Zabrze Sklodowska-Curie, Poland

    Agnieszka Kowalczuk, Marcelina Bochenek, Alicja Utrata-Wesołek, Andrzej Dworak, Barbara Trzebicka & Dawid Szweda

  4. Centre for Burn Treatment in Siemianowice Śląskie, Siemianowice Śląskie Jana Pawla II, Siemianowice Śląskie, Poland

    Agnieszka Klama-Baryła, Justyna GLik, Mariusz Nowak, Marek Kawecki & Aleksander L. Sieroń

  5. Department of Health Sciences, Technical-Humanistic Academy, Bielsko-Biala, Poland

    Agnieszka Klama-Baryła & Marek Kawecki

  6. Department of Chronic Wounds Management Organization, Katowice School of Health Sciences, Katowice Medical University of Silesia, Katowice Medykow, Poland

    Justyna GLik

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  1. Marta Lesiak
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Correspondence to Aleksander L. Sieroń.

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Lesiak, M., Sieroń, Ł., Gutmajster, E. et al. Fibroblast and keratinocyte crosstalk: the effect of a poly(tri[ethylene glycol] ethyl ether methacrylate) thermoresponsive surface on short-term co-culture. Eur J Dermatol 29, 126–140 (2019). https://doi.org/10.1684/ejd.2019.3561

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  • DOI: https://doi.org/10.1684/ejd.2019.3561

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