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Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR)

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European Cytokine Network Aims and scope

Abstract

IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.

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Authors and Affiliations

  1. Cell signaling unit, division of experimental oncology, IRCCS San Raffaele hospital, Milano, Italy

    Maria Giovanna Vilia, Marta Tocchetti, Eleonora Fonte, Ilenia Sana & Marta Muzio

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  1. Maria Giovanna Vilia
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  2. Marta Tocchetti
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  3. Eleonora Fonte
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  4. Ilenia Sana
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  5. Marta Muzio
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Correspondence to Marta Muzio.

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Vilia, M.G., Tocchetti, M., Fonte, E. et al. Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR). Eur Cytokine Netw 28, 63–69 (2017). https://doi.org/10.1684/ecn.2017.0395

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  • DOI: https://doi.org/10.1684/ecn.2017.0395

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