Abstract
We report the recovery of a 7068-nt viral sequence from the “viral fossils” embedded in the genome of Alhagi sparsifolia, a typical desert plant. Although the full viral genome remains to be completed, the putative genome structure, the deduced amino acids and phylogenetic analysis unambiguously demonstrate that this viral sequence represents a novel species of the genus Badnavirus. The putative virus is tentatively termed Alhagi bacilliform virus (ABV). Southern blotting and inverse polymerase chain reaction (PCR) data indicate that the ABV-related sequence is integrated into the A. sparsifolia genome, and probably does not give rise to functional episomal virus. Molecular evidence that the ABV sequence exists widely in A. sparsifolia is also presented. To our knowledge, this is the first endogenous badnavirus identified from plants in the Gobi desert, and may provide new clues on the evolution, geographical distribution as well as the host range of the badnaviruses.
概 要
目 的
新杆状 DNA 病毒的分离与鉴定。
创新点
首次在高寒地区代表性植物——疏叶骆驼刺中发现杆状 DNA 病毒, 为研究杆状 DNA 病毒的进化、 地理分布及寄主范围提供了新证据。
方 法
利用分段聚合酶链式反应 (PCR) 克隆疏叶骆驼刺杆状病毒 (Alhagi bacilliform virus, ABV) 的基因组序列; 通过基因组分析、 序列比对和进化树分析阐明 ABV 的进化地位; 用 Southern 印迹杂交和反向 PCR 分析 ABV 序列与宿主基因组的关系; 并通过 PCR 检测确定 ABV 在我国西北地区疏叶骆驼刺中的分布。
结 论
本研究获得了 7068 nt 的 ABV 基因组序列, 根据基因组结构、 保守序列比对及进化树分析, 推测 ABV 是一种新杆状 DNA 病毒。 分子检测证据表明, ABV 基因组序列已整合进入疏叶骆驼刺基因组中, 但没有产生游离病毒。 此外, 对我国西北 11 个不同地区的疏叶骆驼刺进行 PCR 检测, 结果显示其中 9 个地区的疏叶骆驼刺均含有 ABV 序列, 由此表明 ABV 在我国西北地区的疏叶骆驼刺中广泛存在。
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Acknowledgements
We thank Dr. Wang-bin ZHANG (College of Plant Science, Tarim University, the Xinjiang Uygur Autonomous Region, China) and Dr. Xin-wu PEI (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China) for helping collect the seeds of A. sparsifolia, and Dr. Hong-yan SHAN (Institute of Botany, Chinese Academy of Sciences, Beijing, China) for her helpful suggestion to construct the phylogenetic trees.
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Project supported by the National Natural Science Foundation of China (Nos. 31370181 and 31570146) and the Fujian Natural Science Funds for Distinguished Young Scholar (No. 2014J06008), China
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Li, Yc., Shen, Jg., Zhao, Gh. et al. A novel endogenous badnavirus exists in Alhagi sparsifolia. J. Zhejiang Univ. Sci. B 19, 274–284 (2018). https://doi.org/10.1631/jzus.B1700171
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DOI: https://doi.org/10.1631/jzus.B1700171