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Precise construction of hyaluronic acid (HA)-coated drug-loaded iron oxide metal–organic frameworks: Investigation of cell death mechanism in neuroblastoma carcinoma cells

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Abstract

We constructed an iron (Fe2+) metal–organic framework (MOF) in this study. We loaded SN-38 into the MOF before coating the MOF's surface with hyaluronic acid (SN-38@HA@MOF) to improve its ability to target cancer cells. SN-38@HA@MOF can specifically target cancer tissues, has a high loading efficiency, is biocompatible, has a good monodispersity, has a robust cell absorption capacity, and produces significant intracellular reactive oxygen species levels. The in vitro cytotoxicity of the nanocomposites was investigated in different neuroblastoma cancer cells (SH-SY5Y and IMR 32). Even at a 200 µg/mL dosage, the IC50 range was as high as 85%, indicating that MOF and HA@MOF are non-cytotoxic to non-cancerous cells and have outstanding biocompatibility and low toxicity. Flow cytometric analyses investigated the cell death morphological investigation and mechanism. The adaptive multifunctional SN-38@HA@MOF has the potential to treat targeted neuroblastoma cancer.

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Authors and Affiliations

  1. Department of Oncology, Jiu Jiang No.1 People’s Hospital, No. 48, Taling South Road, Xunyang District, Jiujiang, 332001, China

    Jianhua Deng, Daosheng Li, Yifei Wang, Rui Chen, Denghui Liu & Jindi Peng

  2. Department of Pediatric Surgery, Hunan Children’s Hospital, No.86, Ziyuan Road, Yuhua District, Changsha, 410007, China

    Denghui Liu

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  1. Jianhua Deng
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Contributions

JD and DL: was involved in conceptualisation, methodology, investigation, and writing the original draft. YW and RC: took part in conceptualisation, methodology, investigation, and writing-reviewing editing. DL and JP: participated in resources and writing-reviewing and editing.

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Correspondence to Denghui Liu or Jindi Peng.

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Deng, J., Li, D., Wang, Y. et al. Precise construction of hyaluronic acid (HA)-coated drug-loaded iron oxide metal–organic frameworks: Investigation of cell death mechanism in neuroblastoma carcinoma cells. Journal of Materials Research 39, 489–500 (2024). https://doi.org/10.1557/s43578-023-01244-x

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