Abstract
The advent of bioprinting technology into the tissue engineering field has permitted the attainment of complex-shaped tissue constructs with unprecedented degree of precision and reproducibility, promising for the highly demanded tissue substitutes including vascular grafts. However, most of the bioprinted vascular tissue substitutes still lack multicellular composition and hierarchical complexity of native blood vessels. In this study, a multimaterial bioprinting platform incorporating multiple-channel microfluidic printhead was combined with embedded bioprinting technique for the fabrication of vascular-like constructs. Three different bioink formulations targeting intimal, medial, and adventitial zones of the natural vascular tissues were sequentially extruded from the microfluidic channels of printhead into a hydrogel-nanoclay support bath in a controlled manner to reach the biomimicry of vascular tissues. The results demonstrated the successful deposition of three bioink compositions into distinct zones within hollow structures, which would provide an opportunity for the construction of functional vascular substitutes.
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Acknowledgments
The authors thank Cigdem Bilici for her assistance during GelMA synthesis and characterization. The authors acknowledge Sabanci University and Sabanci University Nanotechnology Research and Application Center (SUNUM). This research is partially supported by the Scientific and Technological Research Council of Turkey (TUBITAK) Grant Number 218S678.
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CD contributed to the design and assembly of multimaterial bioprinting platform, preparation of bioink, bioprinting path planning and bioprinting processes, cellular studies, and confocal microscopy imaging. M.A. contributed cellular studies and confocal microscopy imaging. B.K was responsible for the overall research direction.
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Dikyol, C., Altunbek, M. & Koc, B. Embedded multimaterial bioprinting platform for biofabrication of biomimetic vascular structures. Journal of Materials Research 36, 3851–3864 (2021). https://doi.org/10.1557/s43578-021-00254-x
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DOI: https://doi.org/10.1557/s43578-021-00254-x