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Neuronal Alignment and Outgrowth on Microwrinkled Conducting Polymer Substrates

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Abstract

We report on the results of culturing SH-SY5Y neuron-like cells on PEDOT:PSS wrinkled surfaces fabricated by thermally-induced shrinking of commercial polystyrene sheets. Such smart biointerfaces combine the functional properties of conducting polymers with the topographic patterning at the micro- and sub-microscale, as a result of surface wrinkling. By imposing mechanical constraints during shrinking, anisotropic topographic features are formed, with a spatial periodicity in the range 0.7 - 1.2 μm, tunable by varying the thickness of the PEDOT:PSS thin film. The effectiveness of wrinkled surfaces in enhancing and orientating the outgrowth of neurites is demonstrated by a 42% increase in length and by the 85% of neurites aligned along wrinkles direction (angle 0 <θ < 15°), after 5 days of differentiation. Furthermore, the conductive properties of the PEDOT:PSS film are retained after the surface wrinkling, opening the way for the exploitation of these smart biointerfaces for the electrical stimulation of cells.

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Authors and Affiliations

  1. Center for Micro-BioRobotics IIT@SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, Pontedera, 56025, Italy

    Alberto Bonisoli, Attilio Marino, Gianni Ciofani & Francesco Greco

  2. The BioRobotics Institute, Scuola Superiore Sant’Anna, Viale Rinaldo Piaggio 34, Pontedera, 56025, Italy

    Alberto Bonisoli & Attilio Marino

Authors
  1. Alberto Bonisoli
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  2. Attilio Marino
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  3. Gianni Ciofani
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  4. Francesco Greco
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Bonisoli, A., Marino, A., Ciofani, G. et al. Neuronal Alignment and Outgrowth on Microwrinkled Conducting Polymer Substrates. MRS Online Proceedings Library 1795, 13–18 (2015). https://doi.org/10.1557/opl.2015.558

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  • DOI: https://doi.org/10.1557/opl.2015.558

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