Abstract
The authors have shown recently that the neurite extension by neuronal PC12 cells is greatly impacted by aerogel topography. Indeed, the average neurite length of PC-12 cells grown on aerogels is greater than that in cells cultured on control substrates. Here, the authors report on the first experimental study focused on the design and development of a plasmonic photo-patterning technique for collagen-coated mes-oporous aerogel biomaterials. Herein, the authors have produced specific patterns on silica aerogels by performing precise plasmonic photo-patterning on liquid crystal-coated aerogels. The authors report the methodology employed to create a collagen–liquid crystal gel mixture imprinted with precise plasmonic photo-patterns. PC12 cells plated on these patterns did attach and survive and followed the spatial cues of the pattern to align themselves in a similar pattern.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
M. Hronik-Tupaj, W.K. Raja, M. Tang-Schomer, F.G. Omenetto, and D.L. Kaplan: Neural responses to electrical stimulation on patterned silk films. J. Biomed. Mater. Res. A 101, 2559–2572 (2013).
H. Diekmann and D. Fischer: Parthenolide: a novel pharmacological approach to promote nerve regeneration. Neural Regener. Res. 11, 1566–1567 (2016).
D. Hoffman-Kim, J.A. Mitchel, and R.V. Bellamkonda: Topography, cell response, and nerve regeneration. Annu. Rev. Biomed. Eng. 12, 203–231 (2010).
J.Y. Lim and H.J. Donahue: Cell sensing and response to micro-and nanostructured surfaces produced by chemical and topographic patterning. Tissue Eng. 13, 1879–1891 (2007).
K.J. Lynch, O. Skalli, and F. Sabri: Growing neural PC-12 cell on cross-linked silica aerogels increases neurite extension in the presence of an electric field. J. Funct. Biomater. 9, 30 (2018).
K.J. Lynch, O. Skalli, and F. Sabri: Investigation of surface topography and stiffness on adhesion and neurites extension of PC12 cells on cross-linked silica aerogel substrates. PLoS One 12 (2017).
F. Sabri, M.E. Sebelik, R. Meacham, J.D. Boughter Jr., M.J. Challis, and N. Leventis: In vivo ultrasonic detection of polyurea crosslinked silica aerogel implants. PLoS ONE 8 (2013).
F. Sabri, J.D. Boughter Jr., D. Gerth, O. Skalli, T.-C.N. Phung, G.-R.M. Tamula, and N. Leventis: Histological evaluation of the biocompatibility of polyurea crosslinked silica aerogel implants in a rat model: a pilot study. PLoS ONE 7 (2012).
F. Sabri, J.A. Cole, M.C. Scarbrough, and N. Leventis: Investigation of polyurea-crosslinked silica aerogels as a neuronal scaffold: a pilot study. PLoS ONE 7 (2012).
M. Rodriguez Sala, K.J. Lynch, S. Chandrasekaran, O. Skalli, M. Worsley, and F. Sabri: PC-12 cells adhesion and differentiation on carbon aerogel scaffolds. MRS Commun. 8, 1426–1432 (2018).
J. Hadley, J. Hirschman, B.I. Morshed, and F. Sabri: RF coupling of inter-digitated electrode array on aerogels for in vivo nerve guidance applications. MRS Adv. 4, 1237–1244 (2019).
F. Sabri, D. Gerth, G.-R.M. Tamula, T.-C.N. Phung, K.J. Lynch, and J.D. Boughter Jr.: Novel technique for repair of severed peripheral nerves in rats using polyurea crosslinked silica aerogel scaffold. J. Invest. Surg. 27, 294–303 (2014).
J. Beeckman, K. Neyts, and P.J. Vanbrabant: Liquid-crystal photonic applications. Opt. Eng. 50, 081202 (2011).
P.F. McManamon, P.J. Bos, M.J. Escuti, J. Heikenfeld, S. Serati, H. Xie, and E.A. Watson: A review of phased array steering for narrow-band elec-trooptical systems. Proc. IEEE 97, 1078 (2009).
P. Chen, W. Ji, B.Y. Wei, W. Hu, V. Chigrinov, and Y.Q. Lu: Generation of arbitrary vector beams with liquid crystal polarization converters and vector-photoaligned q-plates. Appl. Phys. Lett. 107, 241102 (2015).
B.Y. Wei, W. Hu, Y. Ming, F. Xu, S. Rubin, J.G. Wang, V. Chigrinov, and Y. Q. Lu: Generating switchable and reconfigurable optical vortices via pho-topatterning of liquid crystals. Adv. Mater. 26, 1590 (2014).
D. Iqbal and M.H. Samiullah: Photo-responsive shape-memory and shape-changing liquid-crystal polymer networks. Materials 6, 116 (2013).
L.T. de Haan, C. Sánchez‐Somolinos, C.M. Bastiaansen, A.P. Schenning, and D.J. Broer: Engineeringof complex order and the macroscopic deformation of liquid crystal polymer networks. Angew. Chem. 124, 12637 (2012).
C. Peng, T. Turiv, R. Zhang, Y. Guo, S.V. Shiyanovskii, Q.H. Wei, J. de Pablo, and O.D. Lavrentovich: Controlling placement of nonspherical (boomerang) colloids in nematic cells with photopatterned director. J. Phys.: Condens. Matter 29, 014005 (2017).
C. Peng, Y. Guo, T. Turiv, M. Jiang, Q.H. Wei, and O.D. Lavrentovich: Patterning of lyotropic chromonic liquid crystals by photoalignment with photonic metamasks. Adv. Mater. 29, 1606112 (2017).
C. Peng, T. Turiv, Y. Guo, Q.-H. Wei, and O.D. Lavrentovich: Command of active matter by topological defects and patterns. Science 354, 882 (2016).
P. van der Asdonk, H.C. Hendrikse, M. Fernandez-Castano Romera, D. Voerman, B.E.I. Ramakers, D.W.P.M. Löwik, R.P. Sijbesma, and P.H.J. Kouwer: Patterning of soft matter across multiple length scales. Adv. Funct. Mater. 26, 2609 (2016).
C. Peng, Y. Guo, C. Conklin, J. Viñals, S.V. Shiyanovskii, Q.-H. Wei, and O. D. Lavrentovich: Liquid crystals with patterned molecular orientation as an electrolytic active medium. Phys. Rev. E 92, 052502 (2015).
K. Gao, H.H. Cheng, A.K. Bhowmik, and P.J. Bos: Thin-film Pancharatnam lens with low f-number and high quality. Opt. Express 23, 26086 (2015).
A. Niv, G. Biener, V. Kleiner, and E. Hasman: Propagation-invariant vecto-rial Bessel beams obtained by use of quantized Pancharatnam–Berry phase optical elements. Opt. Lett. 29, 238 (2004).
Acknowledgments
F.S. would like to thank the FedEx Institute of Technology for partial financial support. C.P. would like to thank the University of Memphis start-up funds.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Rodriguez Sala, M., Peng, C., Skalli, O. et al. Tunable neuronal scaffold biomaterials through plasmonic photo-patterning of aerogels. MRS Communications 9, 1249–1255 (2019). https://doi.org/10.1557/mrc.2019.143
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1557/mrc.2019.143