Abstract
A significant area of research is biomedical applications of nanoparticles which involves efforts to control the physicochemical properties through simple and scalable processes. Gold nanoparticles have received considerable attention due to their unique properties that they exhibit based on their morphology. Gold nanospheres (AuNSs) and nanorods (AuNRs) were prepared with a seed-mediated method followed of polyethylene glycol (PEG)-coating. The seeds were prepared with 0.1 M cetyltrimethyl-ammonium bromide (CTAB), 0.005 M chloroauric acid (HAuCl4), and 0.01 M sodium borohydride (NaBH4) solution. Gold nanoparticles with spherical morphology was achieved by growth by aggregation at room temperature, while to achieve the rod morphology 0.1 M silver nitrate (AgNO3) and 0.1 M ascorbic acid solution were added. The gold nanoparticles obtained by the seed-mediated synthesis have spherical or rod shapes, depending on the experimental conditions, and a uniform particle size. Surface functionalization was developed using polyethylene glycol. Morphology, and size distribution of AuNPs were evaluated by Field Emission Scanning Electron Microscopy. The average size of AuNSs, and AuNRs was 7.85nm and 7.96 x 31.47nm respectively. Fourier transform infrared spectrometry was performed to corroborate the presence of PEG in the AuNPs surface. Additionally, suspensions of AuNSs and AuNRs were evaluated by UV-Vis spectroscopy. Gold nanoparticles were stored for several days at room temperature and it was observed that the colloidal stability increased once gold nanoparticles were coated with PEG due to the shield formed in the surface of the NPs and the increase in size which were 9.65±1.90 nm of diameter for AuNSs and for AuNRs were 29.03±5.88 and 8.39±1.02 nm for length and transverse axis, respectively.
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References
S. Jeon and H. Jeon, “Doxorubicin-loaded oligonucleotide conjugated gold nanoparticles: A promising drug delivery system for ovarian cancer,” Gynecol. Oncol., vol. 159, p. 117, Oct. 2020.
W. Yang, H. Liang, S. Ma, D. Wang, and J. Huang, “Gold nanoparticle based photothermal therapy: Development and application for effective cancer treatment,” Sustainable Materials and Technologies, vol. 22. Elsevier B.V., p. e00109, 01-Dec-2019.
H. Nekounam, Z. Allahyari, S. Gholizadeh, E. Mirzaei, M. A. Shokrgozar, and R. Faridi-Majidi, “Simple and robust fabrication and characterization of conductive carbonized nanofibers loaded with gold nanoparticles for bone tissue engineering applications,” Mater. Sci. Eng. C, vol. 117, p. 111226, Dec. 2020.
L. Zhang, Y. Mazouzi, M. Salmain, B. Liedberg, and S. Boujday, “Antibody-Gold Nanoparticle Bioconjugates for Biosensors: Synthesis, Characterization and Selected Applications,” Biosens. Bioelectron., vol. 165, no. 1, p. 112370, Oct. 2020.
V. G. Flores, “Endocytosis and exocytosis processes of gold nanoparticle with erythrocyte ghosts,” MRS Adv., vol. 5, no. 42, pp. 2169–2172, 2020.
H. Huang et al., “Continuous flow synthesis of ultrasmall gold nanoparticles in a microreactor using trisodium citrate and their SERS performance,” Chem. Eng. Sci., vol. 189, pp. 422–430, Nov. 2018.
C. Daruich De Souza, B. Ribeiro Nogueira, and M. E. C. M. Rostelato, “Review of the methodologies used in the synthesis gold nanoparticles by chemical reduction,” Journal of Alloys and Compounds, vol. 798. Elsevier Ltd, pp. 714–740, 25-Aug-2019.
J. Zhou, J. Ralston, R. Sedev, and D. A. Beattie, “Functionalized gold nanoparticles: Synthesis, structure and colloid stability,” J. Colloid Interface Sci., vol. 331, no. 2, pp. 251–262, 2009.
A. Kraynov and T. E. Muller, “Concepts for the Stabilization of Metal Nanoparticles in Ionic Liquids,” Appl. Ion. Liq. Sci. Technol., pp. 235–260, 2011.
A. S. Fjordbøge, B. Uthuppu, M. H. Jakobsen, S. V. Fischer, and M. M. Broholm, “Mobility of electrostatically and sterically stabilized gold nanoparticles (AuNPs) in saturated porous media,” Environ. Sci. Pollut. Res., 2019.
R. Petry et al., “On the formation of protein corona on colloidal nanoparticles stabilized by depletant polymers,” Mater. Sci. Eng. C, vol. 105, p. 110080, 2019.
A. Franconetti, J. M. Carnerero, R. Prado-Gotor, F. Cabrera-Escribano, and C. Jaime, “Chitosan as a capping agent: Insights on the stabilization of gold nanoparticles,” Carbohydr. Polym., vol. 207, pp. 806–814, 2019.
R. Esquivel et al., “Poly(N-isopropylacrylamide)-coated gold nanorods mediated by thiolated chitosan layer: Thermo-pH responsiveness and optical properties,” E-Polymers, vol. 18, no. 2, pp. 163–174, Feb. 2018.
S. A. Tovar-Cabrera et al., “Hollow Gold Nanoshells Encapsulated in PNIPAM Nanoparticles,” Microsc. Microanal., vol. 24, no. S1, pp. 1794–1795, Aug. 2018.
A. López-Millán et al., “Aqueous-Organic Phase Transfer of Gold and Silver Nanoparticles Using Thiol-Modified Oleic Acid,” Appl. Sci., vol. 7, no. 3, p. 273, Mar. 2017.
C. Chapa-González, A. L. Piñón-Urbina, and P. E. García-Casillas, “Synthesis of controlled-size silica nanoparticles from sodium metasilicate and the effect of the addition of PEG in the size distribution,” Materials (Baselit)., vol. 11, no. 4, Mar. 2018.
M. Lickmichand et al., “In vitro biocompatibility and hyperthermia studies on synthesized cobalt ferrite nanoparticles encapsulated with polyethylene glycol for biomedical applications,” in Materials Today: Proceedings, 2019, vol. 15, pp. 252–261.
J. M. Rabanel, P. A. Piec, S. Landri, S. A. Patten, and C. Ramassamy, “Transport of PEGylated-PLA nanoparticles across a blood brain barrier model, entry into neuronal cells and in vivo brain bioavailability,” J. Control. Release, vol. 328, pp. 679–695, Dec. 2020.
Z. Cao et al., “Enhanced colloidal stability and protein resistance of layered double hydroxide nanoparticles with phosphonic acid-terminated PEG coating for drug delivery,” J. Colloid Interface Sci., vol. 521, pp. 242–251, Jul. 2018.
A. R. M. N. Afrooz, S. T. Sivalapalan, C. J. Murphy, S. M. Hussain, J. J. Schlager, and N. B. Saleh, “Spheres vs. rods: The shape of gold nanoparticles influences aggregation and deposition behavior,” Chemosphere, vol. 91, no. 1, pp. 93–98, 2013.
N. Elahi, M. Kamali, and M. H. Baghersad, “Recent biomedical applications of gold nanoparticles: A review,” Talanta, vol. 184, no. 2018, pp. 537–556, Jul. 2018.
L. A. Dykman, S. A. Staroverov, A. S. Fomin, V. A. Khanadeev, B. N. Khlebtsov, and V. A. Bogatyrev, “Gold nanoparticles as an adjuvant: Influence of size, shape, and technique of combination with CpG on antibody production,” Int. Immunopharmacol., vol. 54, pp. 163–168, 2018.
Y. Kumari et al., “Gold nanoparticles: New routes across old boundaries,” Adv. Colloid Interface Sci., vol. 274, p. 102037, 2019.
B. Nikoobakht and M. A. El-Sayed, “Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method,” Chem. Mater., vol. 15, no. 10, pp. 1957–1962, Apr. 2003.
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Ramírez, S.H.A., García Casillas, P. & González, C.C. Seed-mediated synthesis and PEG coating of gold nanoparticles for controlling morphology and sizes. MRS Advances 5, 3353–3360 (2020). https://doi.org/10.1557/adv.2020.416
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DOI: https://doi.org/10.1557/adv.2020.416