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Direct Bioautographic Detection of Antibacterial Components of Clary Sage and Spearmint Essential Oils

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Summary

The antibacterial effect of the components of clary sage (Salvia sclarea L.) and spearmint (Mentha spicata L. var. crispa (Bentham) Danert) was investigated by means of high-performance thin-layer chromatography-direct bioautography against the Gram-positive soil bacterium Bacillus subtilis (Bs) and Gram-negative bacteria such as a pepper pathogen Xanthomonas euvesicatoria (Xe), a luminescence gene-tagged Arabidopsis pathogen Pseudomonas syringae pv. maculicola (Psm) and a luminescent marine Aliivibrio fischeri (Af). Sclareol, linalool, and linalyl acetate were identified as active components of clary sage and carvone as the antibacterial substance in spearmint. Sclareol inhibited all tested bacteria, linalool and carvone showed antibacterial effect against all Gram-negative strains tested, while linalyl acetate only against Xe and Af. Some minor components of the clary sage essential oil also gave a zone of inhibition when tested on Gram-negative bacterium strains.

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Authors and Affiliations

  1. Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman O. Str. 15, H-1022, Budapest, Hungary

    Ágnes M. Móricz & Péter G. Ott

  2. Department of Pharmacognosy, Medical School, University of Pécs, Rókus u. 2, H-7624, Pécs, Hungary

    Györgyi Horváth

Authors
  1. Ágnes M. Móricz
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  2. Györgyi Horváth
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  3. Péter G. Ott
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Correspondence to Ágnes M. Móricz.

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Móricz, Á.M., Horváth, G. & Ott, P.G. Direct Bioautographic Detection of Antibacterial Components of Clary Sage and Spearmint Essential Oils. JPC-J Planar Chromat 28, 173–177 (2015). https://doi.org/10.1556/JPC.28.2015.2.15

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  • DOI: https://doi.org/10.1556/JPC.28.2015.2.15

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