Abstract
The objective of the present study was to understand the developmental regularity of wheat endosperm cells at different Days After Pollination (DAP) using microscopic and histochemical methods. Resin semi-thin sections of the endosperm and the enzymatically dissociated Starchy Endosperm Cells (SECs) were observed under a light microscope. The results showed that: (1) SECs were irregular-shaped and had two types of starch granules: large oval-shaped A-type starch granules and small spherical B-type starch granules. (2) The growth shape of SECs was referred to as S-curve and the fastest cell growth period was at 16–24 DAP. (3) The largest increase and growth of A-type starch granules were mainly at 4–16 DAP. B-type starch granules increased rapidly after 16 DAP and made up over 90% of the total starch granules in SEC during the late stage of endosperm development. (4) The nuclei of SEC deformed and degenerated during the middle and late stages of endosperm development and eventually disappeared. However, starch granules still increased and grew after the cell nuclei had degenerated. The investigations showed the development regularity of starch endosperm cells and starch granules, thereby improving the understanding of wheat endosperm development.
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Abbreviations
- DAP:
-
Days After Pollination
- PCD:
-
Programmed Cell Death
- SD:
-
Standard Deviation
- SEC:
-
Starch Endosperm Cell
- SEM:
-
Scanning Electron Microscopy
- TTC:
-
2,3,5-Triphenyl-2H-Tetrazolium Chloride
References
Becraft, P.W. 2001. Cell fate specification in the cereal endosperm. Semin. Cell Dev. Biol. 12:387–394.
Becraft, P.W., Gutierrez, M.J. 2012. Endosperm development: dynamic processes and cellular innovations underlying sibling altruism. WIREs Dev. Biol. 1:579–593.
Chen, Y.F., Zhang, J., Xie, P.S., Zhou, W.D., Chen, J.M., Wei, C.X. 2012. Programmed cell death in wheat starchy endosperm during kernel development. Afr. J. Agr. Res. 7:6533–6540.
Hong, S.Y., Park, J.H., Cho, S.H., Yang, M.S., Park, C.M. 2011. Phenological growth stages of Brachypodium distachyon: codification and description. Weed Res. 51:612–620.
Liu, Z., Wang, L.L., Zhou, W.D., Chen, Y.F., Wang, Z. 2011. The surface of the geometric characteristics analysis for rice endosperm starch granules by using Image J. J. of Chinese Electron Microscopy Society 30:466–471. (in Chinese with English abstract)
Olsen, O.A. 1998. Endosperm developments. Plant Cell 10:485–488.
Olsen, O.A. 2004. Nuclear endosperm development in cereals and Arabidopsis thaliana. Plant Cell 16: 215–227.
Sabelli, P.A., Larkins, B.A. 2009a. The development of endosperm in grasses. Plant Physiol. 149:14–26.
Sabelli, P.A., Larkins, B.A. 2009b. The contribution of cell cycle regulation to endosperm development. Sex. Plant Reprod. 22:207–219.
Sabelli, P.A., Liu, Y., Dante, R.A., Lizarraga, L.E., Nguyen, H.N., Brown, S.W., Klingler, J.P., Yu, J., Labrant, E., Layton, T.M., Feldman, M., Larkins, B.A. 2013. Control of cell proliferation, endore duplication, cell size, and cell death by the retinoblastoma-related pathway in maize endosperm. Proc. Natl Acad. Sci. USA 110:1827–1836.
Uhlmann, N.K., Beckles, D.M. 2010. Storage products and transcriptional analysis of the endosperm of cultivated wheat and two wild wheat species. J. Appl. Genet. 51:431–447.
Wang, W.H., Guo, W.S., Fang, M.K., Feng, C.N., Zhu, X.K., Peng, Y.X. 2003. Endosperm cell proliferating and grain filling dynamics in wheat. Acta Agronomica Sinica 29:779–784. (in Chinese with English abstract)
Wang, Z., Gu, Y.J., Wang, H.H., Zheng, Y.K., Chen, Y.H., Zhang, W.H., Wu, X.M. 2012. Investigation of the wheat endosperm cell development. Highlights of Sciencepaper Online 5:1815–1832. (in Chinese with English abstract)
Wei, C.X., Zhang, J., Chen, Y.F., Zhou, W.D., Xu, B., Wang, Y.P., Chen, J.M. 2010. Physicochemical properties and development of wheat large and small starch granules during endosperm development. Acta Physiol. Plant. 32:905–916.
Yang, J.C., Zhang, J.H., Huang, Z.L., Wang, Z.Q., Zhu, Q.S., Liu, L.J. 2002. Correlation of cytokinin levels in the endosperms and roots with cell number and cell division activity during endosperm development in rice. Annals of Botany 90:369–377.
Young, T.E., Gallie, D.R. 1999. Analysis of programmed cell death in wheat endosperm reveals differences in endosperm development between cereals. Plant Mol. Biol. 39:915–926.
Young, T.E., Gallie, D.R. 2000. Programmed cell death during endosperm development. Plant Mol. Biol. 44:283–301.
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Communicated by R.N. Chibbar
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Jing, Y.P., Liu, D.T., Yu, X.R. et al. Development of Endosperm Cells and Starch Granules in Common Wheat. CEREAL RESEARCH COMMUNICATIONS 42, 514–524 (2014). https://doi.org/10.1556/CRC.42.2014.3.14
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DOI: https://doi.org/10.1556/CRC.42.2014.3.14