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Simultaneous Densitometric Determination of Aspirin and Omeprazole by High-Performance Thin-Layer Chromatography

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Summary

A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been developed for the separation and simultaneous estimation of aspirin and omeprazole. Standard and sample solutions of aspirin and omeprazole were applied to aluminum foil silica gel G 60 F254 HPTLC plates. The plates were developed with ethyl acetate–dichloromethane–glacial acetic acid 8:2:0.1 (V/V) as the solvent system. HPTLC detection was performed densitometrically at 241 nm. The linear range was 160–960 ng per band for aspirin and 80–480 ng per band for omeprazole, and the correlation coefficients (R2) were 0.991 and 0.998, respectively. The limits of quantification (LOQ) were 31.89 and 8.01 ng per band, respectively, and the respective limits of detection (LOD) were 10.53 and 2.64 ng per band. Analysis of standard solutions revealed that precision and repeatability were good for both compounds studied.

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, Sinhgad Technical Education Society, Sinhgad Institute of Pharmacy, Narhe, 41, Pune, India

    Arun Maruti Kashid & Omkar Haribhau Kolhe

Authors
  1. Arun Maruti Kashid
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  2. Omkar Haribhau Kolhe
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Correspondence to Arun Maruti Kashid.

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Kashid, A., Kolhe, O. Simultaneous Densitometric Determination of Aspirin and Omeprazole by High-Performance Thin-Layer Chromatography. JPC-J Planar Chromat 32, 501–504 (2019). https://doi.org/10.1556/1006.2019.32.6.8

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  • DOI: https://doi.org/10.1556/1006.2019.32.6.8

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