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High-Performance Thin-Layer Chromatography Method for the Simultaneous Determination of Itopride, Pantoprazole, and Mosapride in Their Formulations and Spiked Human Plasma

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Summary

The combination of itopride (ITP), pantoprazole (PAN), and mosapride (MS) is widely used in the treatment of many gastrointestinal tract (GIT) disorders. For that purpose, a new, simple, precise, accurate, and rapid high-performance thin-layer chromatography (HPTLC) method was developed and validated for the simultaneous determination of ITP, PAN, and MS in their pharmaceutical formulations. The method used Merck HPTLC aluminum plates precoated with silica gel 60 F254 as the stationary phase. The mobile phase consisted of methylene chloride—ethyl acetate—methanol—ammonia (25%) (12:2:0.8:0.2, v/v); this system was found to give compact spot of itopride (R F value of 0.22 ± 0.008), pantoprazole (R F value of 0.41 ± 0.006), and mosapride (R F value of 0.62 ± 0.029). The wavelength of thin-layer chromatography (TLC) scanner was set at 289 nm for both detection and quantitation. The calibration curves were linear over the range of 100–1500 ng spot−1 for ITP and MS, and 70–1500 ng spot−1 for PAN. The detection limits were 32.5, 16.8, and 29.8 for ITP, PAN, and MS, and the quantitation limits were 98.5, 50.3, and 90.5 for ITP, PAN, and MS. The proposed analytical method was validated according to the International Conference on Harmonization (ICH) guidelines, and the results were acceptable. The proposed method has been successfully applied for the determination of the studied drugs in their pharmaceutical preparations as well as in spiked human plasma and it gave excellent percent of recovery. The results showed excellent agreement with the reported method with respect to precision and accuracy.

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References

  1. Y.S. Kim, T.H. Kim, C.S. Choi, Y.W. Shon, S.W. Kim, G.S. Seo, Y.H. Nah, M.G. Choi, S.C. Choi, World J. Gastroenterol. 11 (2005) 4210–4214.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. F. Katagiri, T. Shiga, S. Inoue, Y. Sato, H. Itoh, M. Takeyama, Pharmacology 77 (2006) 115–121.

    Article  CAS  PubMed  Google Scholar 

  3. B. Choudhary, A. Goyal, S.L. Khokra, Int. J. Pharm. Pharm. Sci. 1 (2009) 159–162.

    CAS  Google Scholar 

  4. Z. Santosh, K. Paresh, G. Jitendra, P. Anantwar, B. Sahebrao, Int. J. Drug Deliv. 2 (2010) 340–343.

    Article  Google Scholar 

  5. R.V. Heralgi, C.C. Simpi, N.V. Kalyane, S.R. Karajgi, Asian J. Pharm. (AJP) 2 (2014) 159–165.

    Google Scholar 

  6. X. Hun, Z. Zhang, Sens. Actuators B 131 (2008) 403–410.

    Article  Google Scholar 

  7. A.I. Khan, Z. Khadra, I. Ahmad, L. Khan, A. Khan, M.I. Ullah, Z. Ismail, J. Pharm. Biomed. Anal. 121 (2016) 6–12.

    Article  CAS  PubMed  Google Scholar 

  8. R.V. Thiruvengada, S.T.S. Mohamed, S. Ramkanth, M. Alagusundaram, M. Ganaprakash, K. Chetty, C. Madhusudhana, J. Young Pharm. 2 (2010) 410–413.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. K.R. Gupta, R.B. Chawala, S.G. Wadodkar, Orbital: Electron. J. Chem. 2 (2010) 209–224.

    CAS  Google Scholar 

  10. V. Dighe, R. Sane, S. Menon, H. Tambe, S. Pillai, J. Planar Chromatogr. 19 (2006) 319–323.

    Article  CAS  Google Scholar 

  11. S.P. Sisinthy, S. Duraipandi, N.K. Rao, M.B. Rao, Int. J. Pharm. Pharm. Sci. 7 (2015) 246–249.

    CAS  Google Scholar 

  12. S.S. Singh, M. Jain, K. Sharma, B. Shah, M. Vyas, P. Thakkar, R. Shah, S. Singh, B. Lohray, J. Chromatogr. B 818 (2005) 213–220.

    Article  CAS  Google Scholar 

  13. P. Ptáček, S. Klíma, J. Macek, J. Chromatogr. B 877 (2009) 842–846.

    Article  Google Scholar 

  14. H-W. Lee, J-H. Seo, S-K. Choi, K-T. Lee, Anal. Chim. Acta 583 (2007) 118–123.

    Article  CAS  PubMed  Google Scholar 

  15. A. Bose, U. Bhaumik, A. Ghosh, B. Chatterjee, U.S. Chakrabarty, A.K. Sarkar, T.K. Pal, Chromatographia 69 (2009) 1233–1241.

    Article  CAS  Google Scholar 

  16. Y. Sun, Z. Zhang, Z. Xi, Z. Shi, W. Tian, Anal. Chim. Acta 648 (2009) 174–177.

    Article  CAS  PubMed  Google Scholar 

  17. A. Suganthi, S. John, T.K. Ravi, Indian J. Pharm. Sci. 70 (2008) 366–368.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. F. Ibrahim, J.J. Nasr, Luminescence 31 (2015) 255–263.

    Article  PubMed  Google Scholar 

  19. M.I. Walash, F. Ibrahim, M.I. Eid, S.A.E. Abass, J. Fluoresc. 23 (2013) 1293–1300.

    Article  CAS  PubMed  Google Scholar 

  20. G. Sachs, Pharmacotherapy 17 (1997) 22–37.

    CAS  PubMed  Google Scholar 

  21. K. Basavaiah, U.R.A. Kumar, T. Kalsang, K.B. Vinay, Iranian J. Chem. Chem. Engineer. (IJCCE) 28 (2009) 31–36.

    CAS  Google Scholar 

  22. N.A.E.-R. Nesrin K. Ramadan, M.T. Ragab, B.A. El-Zeany, Spectrochim. Acta A 137 (2015) 463–470.

    Article  Google Scholar 

  23. K. Rajnish, S. Harinder, S. Pinderjit, J. Chem. Pharm. Res. 3 (2011) 113–117.

    Google Scholar 

  24. M. Tanaka, H. Yamazaki, Anal. Chem. 68 (1996) 1513–1516.

    Article  CAS  PubMed  Google Scholar 

  25. Q.B. Cassa, A.L.G. Degania, N.M. Cassianoa, Jr.J. Pedrazolli, J. Chromatogr. B 766 (2002) 153–160.

    Article  Google Scholar 

  26. A.M. Mansour, O.M. Sorour, Chromatographia 53 (2001) S478– S479.

    Article  CAS  Google Scholar 

  27. O. Peres, C.H. Oliveira, R.E. Barrientos-Astigarraga, V.M. Rezende, G.D. Mendes, D. de Nucci, Arzneimittelforschung 54 (2004) 314–319.

    CAS  PubMed  Google Scholar 

  28. R. Magnus, F. Caterina, C. Lars, L. Lars, Eur. J. Gastroenterol. Hepatol. 15 (2003) 1115–1121.

    Article  Google Scholar 

  29. S. Appala Raju, M. Shobha, Asian J. Chem. 15 (2003) 529–531.

    Google Scholar 

  30. A.S. Birajdar, S.N. Meyyanathan, B. Suresh, Int. J. Pharm. Stud. Res. 2 (2011) 29–36.

    Google Scholar 

  31. R. Jainz, K.R.N. Jadon, J. Electrochem. Soc. 155 (2008) F104– F109.

    Article  Google Scholar 

  32. Y.S. Krishnaiah, T.K. Murthy, D.G. Sankar, V. Satyanarayana, Pharmazie 57 (2002) 814–816.

    CAS  PubMed  Google Scholar 

  33. N.V.S. Ramakrishna, K.N. Vishwottam, S. Manoj, M. Koteshwara, J. Chidambara, D.P. Varma, Biomed. Chromatogr. 19 (2005) 539–548.

    Article  CAS  PubMed  Google Scholar 

  34. Y.R. Kumar, J.M. Babu, M.S.P. Sarma, B. Seshidhar, S. Srinivasa Reddy, G. Sudarsan Reddy, K. Vyas, J. Pharm. Biomed. Anal. 32 (2003) 361–368.

    Article  CAS  PubMed  Google Scholar 

  35. F. Qin, L.-Y. Chen, Y.-Y. Ma, D. Wang, J. Liu, X.-M. Lu, F. Li, Yao xue xue bao (Acta Pharmaceutica Sinica) 42 (2007) 882–885.

    CAS  Google Scholar 

  36. Y. Aoki, H. Hakamata, Y. Igarashi, K. Uchida, H. Kobayashi, N. Hirayama, A. Kotani, F. Kusu, J. Chromatogr. B 858 (2007) 135–142.

    Article  CAS  Google Scholar 

  37. M.A. Hegazy, A.M. Yehia, A.A. Mostafa, Drug Test. Anal. 4 (2012) 104–115.

    Article  CAS  PubMed  Google Scholar 

  38. D. Aleksandrova, Y. Scripinets, A. Yegorova, Acta Pol. Pharm. Drug Res. 66 (2009) 605–610.

    CAS  Google Scholar 

  39. K.R. Gupta, R.B. Chawala, S.G. Wadodkar, Orbital: Electron. J. Chem. 2 (2010) 209–224.

    CAS  Google Scholar 

  40. G. Darshan, B. Prachi, J. Reshma, R. Sadhana, Int. J. Pharm. Sci. Res. 5 (2014) 907–912.

    Google Scholar 

  41. International Conference on Harmonisation, Harmonised Tripartite Guideline: Validation of Analytical Procedures: Text and Methodology Q2(R1), Geneva, 2005.

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Authors and Affiliations

  1. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Port Said University, Port Said, Egypt

    Roshdy E. Saraya

  2. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt

    Randa A. Abdel Salam & Ghada M. Hadad

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  1. Roshdy E. Saraya
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  2. Randa A. Abdel Salam
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  3. Ghada M. Hadad
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Correspondence to Roshdy E. Saraya.

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Saraya, R.E., Abdel Salam, R.A. & Hadad, G.M. High-Performance Thin-Layer Chromatography Method for the Simultaneous Determination of Itopride, Pantoprazole, and Mosapride in Their Formulations and Spiked Human Plasma. JPC-J Planar Chromat 30, 299–306 (2017). https://doi.org/10.1556/1006.2017.30.4.10

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