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An Endangered Medicinal Plant, Plumbago zeylanica L. and Its Substitute, Dyerophytum indicum (Gibbs ex Wight) Kuntze: A Comparative HPTLC, HPLC Study, and Screening of Antioxidant Activity

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Summary

Plumbago zeylanica L. (PZ) is a significant medicinal plant in Ayurveda, so is used for the treatment of various disorders. The main active part of this plant is the root, and due to its inherent uses, it has been exploited hence becoming an endangered species. Dyerophytum indicum (Gibbs ex Wight) Kuntze (DI), mainly considered as a substitute of PZ, is also an important folklore medicine, used in many health problems. Both plants are much similar in their physical as well as chemical properties. However, an effective validation is required before declaration of substitution. In the present study, quantitative and qualitative estimations were performed on both plants with the help of modern analytical techniques. Simultaneous high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) and quantitative determinations of β-sitosterol have been performed for comparing both plants, i.e., PZ and DI. HPTLC fingerprinting analysis was also performed comparatively in different plant parts of PZ and DI. Successive extracts from different plant parts were evaluated for TLC separation profile of secondary metabolites. A comparative polyphenolic content- and antioxidant screening was evaluated to check the free radical scavenging effect of both plants (leaf, stem, and root) in comparison with the standards gallic and ascorbic acid.

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Authors and Affiliations

  1. Department of Life Sciences, Suresh Gyan Vihar University, 302017, Mahal Jagatpura, Jaipur, India

    Richa Tyagi & Gaurav Sharma

  2. Department of Biotechnology, JECRC University, Sitapura Industrial Area Extn, Ramchandrapura, Vidhani Village, 303905, Jaipur, India

    Ekta Menghani

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  1. Richa Tyagi
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  2. Ekta Menghani
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  3. Gaurav Sharma
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Correspondence to Richa Tyagi.

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Tyagi, R., Menghani, E. & Sharma, G. An Endangered Medicinal Plant, Plumbago zeylanica L. and Its Substitute, Dyerophytum indicum (Gibbs ex Wight) Kuntze: A Comparative HPTLC, HPLC Study, and Screening of Antioxidant Activity. JPC-J Planar Chromat 30, 17–24 (2017). https://doi.org/10.1556/1006.2017.30.1.2

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