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Chromatographic Approach for the Evaluation of Radical-Scavenging Activity Using a New Time-Monitoring Image Analysis Method

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Summary

A thin-layer chromatographic (TLC) procedure combined with digital image processing analysis was developed for quantitative time-monitoring of the radical- scavenging activity. Reaction of 2,2-diphenyl-1-picrylhydrazyl (DPPH•) with reference antioxidants and selected catecholamine compounds was monitored on RP-18 chromatographic plates using dot-blot technique and different concentrations of standards. Images of the chromatographic plate were recorded after background staining every 5 min for 1 h using a TLC scanner device. ImageDecipher TLC software program was used to convert image data into chromatograms, from which spot area as a function of reaction time was easily monitored for different amounts of antioxidant in all cases. For a comparative evaluation of radical-scavenging activity, all the time-acquired images were documented as a new red, green, and blue (RGB) image after conversion to different color values. Based on the chromatogram digitization results, TLC—time-monitored radical-scavenging profiles of the investigated compounds were obtained. By the developed methodology, different image processing and image analysis procedures were proposed and originally applied for the first time in TLC—time-monitoring of radical-scavenging activity evaluation.

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Authors and Affiliations

  1. Babes-Bolyai University, Faculty of Chemistry and Chemical Engineering, Department of Chemistry, 11 Arany Janos Str., Cluj-Napoca, 400028, Romania

    Dorina Casoni & Costel Sârbu

Authors
  1. Dorina Casoni
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  2. Costel Sârbu
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Correspondence to Costel Sârbu.

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Casoni, D., Sârbu, C. Chromatographic Approach for the Evaluation of Radical-Scavenging Activity Using a New Time-Monitoring Image Analysis Method. JPC-J Planar Chromat 29, 299–305 (2016). https://doi.org/10.1556/1006.2016.29.4.8

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  • DOI: https://doi.org/10.1556/1006.2016.29.4.8

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