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Molecular characterisation and functional analysis of a cytochrome P450 gene in cotton

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Abstract

Verticillium wilt causes devastating loss of yield and quality in many crops, including cotton. To determine the molecular mechanism of resistance to verticillium wilt in cotton, we isolated a new cytochrome P450 gene, CYP94C1, and analysed its function. We obtained the complete open reading frame, which encodes a protein of 500 amino acids. The results of the functional analysis showed that resistance to verticillium wilt was enhanced when the gene was silenced using the virus-induced gene silencing (VIGS) method in cotton. GbCYP94C1-overexpressing Arabidopsis was more susceptible to Verticillium dahliae compared with the wild-type. In addition, GbCYP94C1 played a role in plant wound responses and in the expression of certain jasmonic acid (JA) signalling pathway. The results suggest that GbCYP94C1 plays a role in verticillium wilt resistance in cotton via the JA signalling pathway.

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Abbreviations

VIGS:

virus-induced gene silencing

JA:

jasmonic acid

qRT-PCR:

quantitative polymerase chain reaction

MeJA:

methyl jasmonate

V. dahlia :

Verticillium dahlia

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  1. Contributed equally to this work

Authors and Affiliations

  1. State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, China

    Kexue Zhou, Lu Long, Quan Sun, Weina Wang, Wei Gao, Chaowei Cai, Jianchuan Mo, Jieru Cheng, Xiangrui Zhang, Yujia Liu, Chen Miao, Xiao Zhang & Yingfan Cai

  2. College of Bioinformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065, China

    Quan Sun & Weina Wang

  3. State Key Laboratory of Cotton Biology, Cotton Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang, 455000, China

    Xiongming Du, Yuzhen Shi & Youlu Yuan

  4. Kaifeng Academy of Agriculture and Forestry, Kaifeng, 475004, China

    Zongyan Chu

Authors
  1. Kexue Zhou
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  2. Lu Long
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  3. Quan Sun
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  4. Weina Wang
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  5. Wei Gao
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  7. Chaowei Cai
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  8. Jianchuan Mo
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  9. Jieru Cheng
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  10. Xiangrui Zhang
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  13. Chen Miao
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  14. Yuzhen Shi
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  15. Youlu Yuan
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  16. Xiao Zhang
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  17. Yingfan Cai
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Correspondence to Youlu Yuan or Yingfan Cai.

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Zhou, K., Long, L., Sun, Q. et al. Molecular characterisation and functional analysis of a cytochrome P450 gene in cotton. Biologia 72, 43–52 (2017). https://doi.org/10.1515/biolog-2017-0003

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