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Mitochondrial genotyping of Fascioloides magna from Bavaria, Germany

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Abstract

In last few years, a great effort has been made to understand genetic interrelationships of European and North American populations of giant liver fluke Fascioloides magna (Trematoda, Fasciolidae). In Europe, spatial distribution of this parasite is evidently dynamic and ongoing process since new F. magna populations have constantly been emerging. Most recently occurrence of F. magna in red deer (Cervus elaphus), sika deer (Cervus nippon), fallow deer (Dama dama), roe deer (Capreolus capreolus), and wild boar (Sus scrofa) was reported from north-eastern Bavaria in Germany. Fascioloides magna specimens collected from those hosts were genotyped using two mitochondrial regions; cytochrome c oxidase (cox1) and nicotinamide dehydrogenase (nad1). Results were compared with reference mitochondrial haplotypes of previously characterized European F. magna populations from northern Italy, Czech Republic/Poland, and the Danube floodplain forests. The study revealed genetic uniformity of F. magna specimens from Germany with flukes from the Czech focus, what indicated that fascioloidosis has been spreading beyond well established Czech focus into neighbouring countries.

Note Nucleotide sequence data reported in this paper are available in the GenBank™, EMBL and DDBJ databases under theaccession numbers KY793001-2.

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References

  • Chroustová E., Hůlka J., Jaroš J. 1980. Prevence a terapie fascioloidózy skotu bithionolsulfoxidem. Veterinární Medicína (Praha), 25, 557–563. (In Czech)

    PubMed  Google Scholar 

  • Chroust K., Chroustová E. 2004. Motolice obrovská (Fascioloides magna) u spárkaté zvěře v jihočeských lokalitách. Veterinářství, 54, 296–304. (In Czech)

    Google Scholar 

  • Demiaszkiewicz A.W., Kuligowska I., Pyziel A.M., Lachowicz J., Kowalczyk R. 2015. Extension of occurrence area of the American fluke Fascioloides magna in south-western Poland. Annals of Parasitology, 61, 93–96.

    PubMed  Google Scholar 

  • Demiaszkiewicz A.W., Pyziel A.M., Kuligowska I., Lachowicz J. 2016. Fascioloides magna pasożytem jeleni w Borach Dolnośląskich. Medycyna Weterynaryjna, 72, 110–112. (In Polish)

    Google Scholar 

  • Erhardová-Kotrlá B. (Ed.). 1971. The occurrence of Fascioloides magna (Bassi, 1875) in Czechoslovakia. Czechoslovak Academy of Sciences, Prague, pp. 155

    Google Scholar 

  • Houzka M., Piekarska J., Podkowik M., Gorczykowski M., Bania J. 2016. Morphology and molecular study of Fascioloides magna — a growing threat to cervids (Cervidae) in Poland. Journal of Veterinary Research, 60, 435–439

    Article  Google Scholar 

  • Juhásová L., Králová-Hromadová I., Bazsalovicsová E., Minárik G., Štefka J., Mikulíček P., et al. 2016a. Population structure and dispersal routes of an invasive parasite, Fascioloides magna, in North America and Europe. Parasites & Vectors, 9, 547. DOI: 10.1186/s13071-016-1811-z

    Article  Google Scholar 

  • Juhásová L., Bazsalovicsová E., Králová-Hromadová I., Karamon J. 2016b. A genetic structure of novel population of Fascioloides magna from Poland, Podkarpackie Province, indicates an expanding second European natural focus of fascioloidosis. Acta Parasitologica, 61, 790–795. DOI: 10.1515/ap-2016-0109

    Article  PubMed  Google Scholar 

  • Karamon J., Larska M., Jasik A., Sell B. 2015. First report of the giant liver fluke (Fascioloides magna) infection in farmed fallow deer (Dama dama) in Poland — pathomorphological changes and molecular identification. Bulletin of the Veterinary Institute in Puławy, 59, 339–344. DOI: 10.1515/bvip-2015-0050

    Article  Google Scholar 

  • Kašný M., Beran L., Siegelová V., Siegel T., Leontovyč R., Beránková K., et al. 2012. Geographical distribution of the giant liver fluke (Fascioloides magna) in the Czech Republic and potential risk of its further spread. Veterinární Medicína (Prague), 57, 101–109

    Article  Google Scholar 

  • Králová-Hromadová I., Špakulová M., Horáčková E., Turčeková L., Novobilský A., Beck R., et al. 2008. Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. Journal of Parasitology, 94, 58–67. DOI: 10.1645/GE-1324.1

    Article  PubMed  Google Scholar 

  • Králová-Hromadová I., Bazsalovicsová E., Štefka J., Špakulová M., Vávrová S., Szemes T., et al. 2011. Multiple origins of European populations of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), a liver parasite of ruminants. International Journal for Parasitology, 41, 373–383. DOI: 10.1016/j.ijpara.2010.10.010

    Article  PubMed  Google Scholar 

  • Králová-Hromadová I., Bazsalovicsová E., Demiaszkiewicz A.W. 2015. Molecular characterization of Fascioloides magna (Trematoda: Fasciolidae) from south-western Poland based on mitochondrial markers. Acta Parasitologica, 60, 544–547. DOI: 10.1515/ap-2015-0077

    Article  PubMed  Google Scholar 

  • Králová-Hromadová I., Juhásová L., Bazsalovicsová E. (Eds). 2016. The giant liver fluke, Fascioloides magna: Past, present and future research. Springer International Publishing, Heidelberg, Germany, pp. 105

    Google Scholar 

  • Novobilský A., Horáčková E., Hirtová L., Modrý D., Koudela B. 2007. The giant liver fluke Fascioloides magna (Bassi 1875) in cervids in the Czech Republic and potential of its spreading to Germany. Parasitology Research, 100, 549–553. DOI: 10.1007/s00436-006-0299-4

    Article  PubMed  Google Scholar 

  • Plötz C., Rehbein S., Bamler H., Reindl H., Pfister K., Scheuerle M.C. 2015. Fascioloides magna — epizootiology in a deer farm in Germany. Berliner und Münchener Tierärztliche Wochenschrift, 128, 177–182.

    PubMed  Google Scholar 

  • Pybus M.J. 2001. Liver flukes. In: (Eds W.M. Samuel, M.J. Pybus and A.A. Kocan) Parasitic Diseases of Wild Mammals, 2nd edn. Iowa State University Press, Ames, pp. 121–149

    Chapter  Google Scholar 

  • Pyziel A.M., Demiaszkiewicz A.W., Kuligowska I. 2014. Molecular identification of Fascioloides magna (Bassi, 1875) from red deer from south-western Poland (Lower Silesian Wilderness) on the basis of internal transcribed spacer 2 (ITS-2). Polish Journal of Veterinary Sciences, 17, 523–525. DOI: 10.2478/pjvs-2014-0077

    Article  PubMed  CAS  Google Scholar 

  • Rehbein S., Hamel D., Reindl H., Visser M., Pfister K. 2012. Fascioloides magna and Ashworthius sidemi — two new parasites in wild ungulates in Germany. In: Program and Abstracts of the XI European Multicolloquium of Parasitology (EMOP XI), 25–29 July 2012, Cluj-Napoca, Romania, p. 565

    Google Scholar 

  • Salomon S. 1932. Fascioloides magna bei deutschem Rotwild. Berliner und Münchener Tierärztliche Wochenschrift, 48, 627–628. (In German)

    Google Scholar 

  • Ślusarski W. 1955. Studia nad europejskimi przedstawicielami przywry Fasciola magna (Bassi, 1875) Stiles, 1894. Acta Parasitologica Polonica, 3, 1–59. (In Polish)

    Google Scholar 

  • Ullrich K. 1930. Über das Vorkommen von seltenen oder wenig bekannten Parasiten der Säugetiere und Vögel in Böhmen und Mähren. Prager Archiv für Tiermedizin, 10, 19–43. (In German)

    Google Scholar 

  • Werle E., Schneider C., Renner M., Volker M., Fiehn W. 1994. Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Research, 22, 4354–4355

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Záhoř Z., Prokeš C., Vítovec L. 1966. Nález vajíček motolice Fascioloides magna (Bassi, 1875) a fascioloidózních změn v játrech skotu. Veterinární Medicína (Praha), 39, 397–404. (In Czech)

    Google Scholar 

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Authors and Affiliations

  1. Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovakia

    Eva Bazsalovicsová, Ludmila Juhásová & Ivica Králová-Hromadová

  2. Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8-12, D-83101, Rohrdorf, Germany

    Steffen Rehbein

Authors
  1. Eva Bazsalovicsová
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  2. Ludmila Juhásová
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  3. Ivica Králová-Hromadová
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  4. Steffen Rehbein
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Correspondence to Ivica Králová-Hromadová.

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Bazsalovicsová, E., Juhásová, L., Králová-Hromadová, I. et al. Mitochondrial genotyping of Fascioloides magna from Bavaria, Germany. Acta Parasit. 62, 870–874 (2017). https://doi.org/10.1515/ap-2017-0105

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