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Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens

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Abstract

Three recombinant antigens viz. arginine kinase, cathepsin L-1 and TES-26 of Toxocara canis were expressed in Escherichia coli and evaluated for their potential in the detection of T. canis larval infection in human in immunoglobulin G-enzyme linked immunosorbent assay (IgG-ELISA). Results of the IgG-ELISA with the above recombinant antigens were confirmed with commercially available IgG detection kit for T. canis infection used as a standard test. All three recombinant antigens were 100% sensitive in the detection of positive cases (n = 6) of T. canis infection in human and were screened for their cross-reactivity in human patients with history of Toxoplasma gondii, Plasmodium vivax, Entamoeba histolytica, hydatid and hookworm infections. The recombinant TES-26 antigen showed higher specificity and cross-reacted with T. gondii infection sera only. However, arginine kinase and cathepsin L-1 recombinant antigens showed cross-reactions with sera of patients infected with T. gondii, P. vivax and E. histolytica but not with the patient sera infected with hydatid and hookworm. These results show that recombinant TES-26 is a potential diagnostic candidate antigen for human toxocarosis caused by migrating T. canis larvae.

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Authors and Affiliations

  1. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar-India, India

    Opinder K. Raina, Dinesh Chandra, Anandanarayanan Arun, Ajayta Rialch, Hniang Lalrinkima & Subhamoy Samanta

  2. Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Kerala, India

    Anju Varghese & Reghu Ravindran

  3. Department of Microbiology, All Indian Institute of Medical Sciences, New Delhi, India

    Bijay R. Mirdha

  4. Department of Veterinary Parasitology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India

    Naresh H. Kelawala, Jayesh B. Solanki, Niranjan Kumar & Rohan N. Kelawala

Authors
  1. Anju Varghese
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  2. Opinder K. Raina
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  3. Dinesh Chandra
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  4. Bijay R. Mirdha
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  5. Naresh H. Kelawala
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  6. Jayesh B. Solanki
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  7. Niranjan Kumar
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  8. Reghu Ravindran
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  9. Anandanarayanan Arun
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  10. Ajayta Rialch
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  11. Hniang Lalrinkima
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  12. Rohan N. Kelawala
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  13. Subhamoy Samanta
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Correspondence to Anju Varghese.

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Varghese, A., Raina, O.K., Chandra, D. et al. Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens. Acta Parasit. 62, 775–778 (2017). https://doi.org/10.1515/ap-2017-0093

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  • DOI: https://doi.org/10.1515/ap-2017-0093

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