Abstract
The aim of this study was to evaluate the occurrence of Toxoplasma gondii in backyard pigs destined for human consumption in Pernambuco state, Brazil. Blood and tissue samples (liver, heart, brain, lung and diaphragm) were collected from 224 pigs from legal slaughterhouses and tested for T. gondii infection. Antibodies to T. gondii were found in the sera of 37.9% (85/224) by using the immunofluorescence antibody test (cut-off–1:64). Tissue samples from seropositive pigs were bioassayed in mice. Tissue samples from seropositive pigs and from mice of the bioassay were submitted to histopathology, immunohistochemistry, polymerase chain reaction (PCR) and sequencing; 14.1% of pig tissue samples and 27.7% of bioassayed mouse samples were positive for T. gondii DNA, but all pig and mouse tissues were negative in histopathology analysis and immunochemistry. By using a risk assessment questionnaire, there was significant difference (p<0.001) in seroprevalence of 21.2% (reproducer) and 3.1% (finishing pig). These data serve as indicative of the sanitary conditions and risk of T. gondii infection for backyard pigs. Preventive measures must be implemented by health services to avoid toxoplasmosis human cases due to ingestion of pig meat.
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Berger-Schoch A., Herrmann D., Schares G., Müller N., Bernet D., Doherr M., et al. 2010. Incidence and genotypes of Toxopla-sma gondii in the muscle of sheep, cattle and pigs in Switzerland. Veterinary Parasitology, 1, 290–97. DOI: 10.1016/j.vetpar.2010.11.046
Bezerra R.A., Carvalho F.S., Guimarães L.A., Rocha D.S., Maciel B.M., Wenceslau A.A., et al. 2012. Genetic characterization of Toxoplasma gondii isolation from pigs intended for human consumption in Brazil. Veterinary Parasitology, 189, 153–61. DOI: org/10.1016/j.vetpar.2012.04.036
Cademartori B.G., Santos L.M.J.F., Oliveira A.F.C., Quevedo A.P., Oliveira A.P.A., Ramos T.S., et al. 2014. Isolation and pathogenicity of Toxoplasma gondii in naturally infected (rustic farm) pigs in southern Brazil. Veterinary Parasitology, 203, 207–21. DOI: 10.1016/j.vetpar.2014.02.009
Camargo M.E. 1974. Introduction to immunofluorescence technique. Revista Brasileira de Patologia Clínica, 10, 143–171 (In Portuguese)
Demar M., Ajzenberg D., Maubon D., Djossou F., Panchoe D., Punwasi W., et al. 2007. Fatal outbreak of human toxoplasmosis along the Maroni River: epidemiological, clinical and parasitological aspects, Clinical Infectious Diseases, 45:88–95. DOI: 10.1086/521246. [PubMed] [Cross Ref]
Dubey J.P. Long-term persistence of Toxoplasma gondii in tissues of pigs inoculated with T. gondii oocysts and effect of freezing on viability of tissue cysts in pork. 1988. American Journal Veterinary Research, 49, 910–3. PMID:3400928
Dubey J.P., Weigel R.M., Siegel A.M., Thulliezt P., Kitron U.D., Mitchell M.A., et al. 1995. Sources and reservoirs of Toxoplasma gondii infection on 47 swine farms in Illinois. Journal Para-sitology, 81, 723–29. PubMed: 7472862
Dubey J.P. Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues. 1998. Veterinary Para-sitology, 74, 75–77. DOI: 10.1016/S0304-4017(97)00135-0
Dubey J.P., Hill D.E., Jones J.L., Hightower A.W., Kirkland E., Roberts J.M., et al. 2005. Prevalence of viable Toxoplasma gon-dii in beef, chicken, and pork from retail meat stores in the United States: risk assessment to consumers. Journal Parasit-ology, 91, 1082–93. DOI: org/10.1645/GE-683.1
Dubey, J.P. 2009. Toxoplasmosis in pigs — The last 20 years. Veterinary Parasitology, 164, 89–103. DOI: org/10.1016/j.vet-par.2009.05.018
Dubey J.P. (Ed.) 2010. Toxoplasmosis of animals and humans. Second Edition. Florida, (CRC Press). pp.336
Dubey, J.P., Lago, E.G., Gennari, S.M., Su, C., Jones, J.L., 2012. Toxoplasmosis in humans and animals in Brazil: high prevalence, high burden of disease, and epidemiology. Parasitology. 139, 1375–1424
Feitosa T.F., Vilela V.L.R., Melo L.R.B., Neto J.L.A., Souto D.V.O., Morais D.F., et al. 2014. Toxoplasma gondii and Neospora caninum in slaughtered pigs from Northeast, Brazil. Veterinary Parasitology, 202: 305–309. DOI: 10.1016/j.vetpar.2014.03.015
Fernandes E.F.T.S., Fernandes M.F.T.S., Kim P.C.P., de Albuquerque P.P.F., Neto O.L.S., Santos A.S., et al. 2012. Study of Toxoplasma gondii in slaughtered swine in the state of Per-nambuco, Brazil. Journal Parasitology, 98:690–91. DOI: 10.1645/GE-3032.1
Galiza G.J.N., Tochetto C., Rosa F.B., Panziera W., Silva T.M., Caprioli R.A., et al. 2014. Usage of three immunohistochemical methods in the detection of aspergillosis and zygomycosis in animals. Pesquisa Veterinária Brasileira, 34, 637–42. DOI: org/10.1590/S0100-736X2014000700005
Homan W.L., Vercammen M., de Braekeleer J., Verschueren H. 2000. Identification of a 200 — to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. International Journal Parasitology, 30, 69–75. DOI: org/10.1016/S0020-7519(99)00170-8
Landis J.R., Koch G.G. 1977. The measurement of observer agreement for categorical data. Biometrics, 33, 159–74
Luna L.G. (Ed.). 1968. Manual of histologic staining methods of the armed forces institute of pathology. New York, (McGraw-Hill Book Company). (In Portuguese)
Oliveira P.A., Oliveira F.C., Faria L.M.J., Cademartori B.G., Pereira C.M., Coelho A.C.B., et al. 2014. Patogenicidade e virulência de Toxoplasma gondii isolado de suínos de criação artesanal no sul do Brasil. Pesquisa Veterinária Brasileira, 34, 1186–90. DOI: org/10.1590/S0100-736X2014001200007
Piassa F.R., Araújo J.B., Rosa R.C., Mattei R.J., Silva R.C., Langoni H., et al. 2010. Prevalence and risk factors for Toxoplasma gon-dii infection in certified and non-certified pig breeding farms in the Toledo microregion, PR, Brazil. Revista Brasileira Parasi-tologia Veterinária, 19, 152–56. DOI: org/10.1590/S1984-29612010000300005
Silva A.V., Da Silva R.C., Zamprogna T.O. 2010. Toxoplasma gon-dii in pigs with emphasis on the Brazilian contribution. Scince Medical, 20: 120–130. (In Portuguese)
Swierzy I.J., Muhammad M., Kroll J., Abelmann A., Tenter A.M., Luder C.G. 2014. Toxoplasma gondii within skeletal muscle cells: a critical interplay for food-borne parasite transmission. International Journal Parasitology, 44, 91–8. DOI: 10.1016/j.ijpara.2013.10.001. [PubMed] [Cross Ref]
Torgerson P.R., Silva N.R., Fevre E.M., Kasuga F., Rokni M.B., Zhou X.N., et al. 2014. The global burden of foodborne parasitic diseases: an update. Trends Parasitology, 30, 20–6. DOI: 10.1016/j.pt.2013.11.002.[PubMed] [Cross Ref]
Thrusfield M.V. (Ed.). 2004. Veterinary Epidemiology. Second Edition. São Paulo, (Roca), pp. 556. (In Portuguese)
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Samico-Fernandes, E.F.T., Samico-Fernandes, M.F.T., de Albuquerque, P.P.F. et al. Toxoplasma gondii in backyard pigs: seroepidemiology and mouse bioassay. Acta Parasit. 62, 466–470 (2017). https://doi.org/10.1515/ap-2017-0054
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DOI: https://doi.org/10.1515/ap-2017-0054