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Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo

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Abstract

Cryptosporidium is an important protozoan parasite affecting HIV/AIDS patients. To determine the prevalence and the species of Cryptosporidium spp by developing a rapid and efficient real-time PCR-RFLP test. A cross-sectional study was conducted among HIV-infected adults from Kinshasa, the Democratic Republic of Congo. Stool specimens were examined by microscopic evaluation and real-time PCR-RFLP. Out of 242 HIV-infected adults, 10 (4.1%) cases of Cryptosporidium were identified by microscopic examination. Using PCR-RFLP, the prevalence of Cryptosporidium spp was 5.4% (n = 13). All the 13 cases of Cryptosporidium spp had the stage of AIDS of HIV infection. Extracted DNA was amplified by nested PCR targeting a 1030-bp fragment of the 18s RNA gene. RFLP analysis identified one C. parvum, four C. hominis and one non determined Cryptosporidium. The capacity to detect C. parvum, C. hominis and non-determined Cryptosporidium was present among our HIV-infected patients.

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Authors and Affiliations

  1. Department of Tropical Medicine, University Clinic of Kinshasa, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo

    Roger D. Wumba, Josué Zanga, Kennedy M. Mbanzulu & Aimé K. Kahindo

  2. Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo

    Roger D. Wumba, Josué Zanga & Kennedy M. Mbanzulu

  3. Division of Infectious Diseases, Department of Internal Medicine, University Hospital of Kinshasa, School of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo

    Madone N. Mandina & Mathilde B. Ekila

  4. Division of Hemato-oncology and Nephrology, Department of Paediatrics, University Hospital of Kinshasa, School of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo

    Michel N. Aloni

Authors
  1. Roger D. Wumba
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  2. Josué Zanga
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  3. Kennedy M. Mbanzulu
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  4. Madone N. Mandina
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  5. Aimé K. Kahindo
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  6. Michel N. Aloni
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  7. Mathilde B. Ekila
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Correspondence to Roger D. Wumba.

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Wumba, R.D., Zanga, J., Mbanzulu, K.M. et al. Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo. Acta Parasit. 60, 638–644 (2015). https://doi.org/10.1515/ap-2015-0090

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  • DOI: https://doi.org/10.1515/ap-2015-0090

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