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Biomaterials for molecular electronics development of optical biosensor for retinol

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Abstract

Molecular electronics involves expertise from several branches of science. Various biomaterials and electronics are involved in the fabrication of such devices. While passive biomaterials are involved in anchoring the active biomolecules, the latter are involved in switching and/or signal transduction. In the present investigation we have used a glass-capillary-based approach to design a biosensor for retinol. The sensing element is retinol-binding protein (RBP). The affinity of retinoic-acid-horseradish peroxidase (conjugate) to RBP is tested using a surface plasmon resonance technique. A simple photomultiplier-tube-based system is exploited to monitor the chemiluminescent signal generated upon reaction of hydrogen peroxide and luminol with the conjugate bound to RBP. The photomultiplier tube is directly coupled to a computer for data logging.

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Authors and Affiliations

  1. Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, 221 00, Lund, Sweden

    Kumaran Ramanathan, Juraj Svitel, Anatoli Dzgoev & Bengt Danielsson

  2. Centre for Protein Engineering and Biomedical Research, The Voluntary Health Services, 600 113, Adyar, Madras, India

    P. V. Sundaram

Authors
  1. Kumaran Ramanathan
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  2. Juraj Svitel
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  3. Anatoli Dzgoev
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  4. P. V. Sundaram
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  5. Bengt Danielsson
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Correspondence to Kumaran Ramanathan.

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Ramanathan, K., Svitel, J., Dzgoev, A. et al. Biomaterials for molecular electronics development of optical biosensor for retinol. Appl Biochem Biotechnol 96, 287–301 (2001). https://doi.org/10.1385/ABAB:96:1-3:287

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