Amyloid Proteins

1. Front Matter

   Pages i-xiv

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2. In Vitro Assays

   1. Front Matter

      Pages 1-1

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   2. Preparation of Aggregate-Free,Low Molecular Weight Amyloidβ for Assembly and Toxicity Assays

      • Gal Bitan, David B. Teplow

      Pages 3-9

   3. Determination of Peptide Oligomerization State Using Rapid Photochemical Crosslinking

      • Sabrina S. Vollers, David B. Teplow, Gal Bitan

      Pages 11-18

   4. In Vitro Preparation of Prefibrillar Intermediates of Amyloid-β and α Synuclein

      • Hilal A. Lashuel, Dolors Grillo-Bosch

      Pages 19-33

   5. Purification of Recombinant Tau Protein and Preparation of Alzheimer-Paired Helical Filaments In Vitro

      • Stefan Barghorn, Jacek Biernat, Eckhard Mandelkow

      Pages 35-51

   6. Cyclic Amplification of Protein Misfolding and Aggregation

      • Paula Saá, Joaquín Castilla, Claudio Soto

      Pages 53-65

   7. X-Ray Diffraction Studies of Amyloid Structure

      • O. Sumner Makin, Louise C. Serpell

      Pages 67-80

   8. Molecular Electron Microscopy Approaches to Elucidating the Mechanisms of Protein Fibrillogenesis

      • Hilal A. Lashuel, Joseph S. Wall

      Pages 81-101

   9. Time -Lapse Atomic Force Microscopy in the Characterization of Amyloid-Like Fibril Assembly and Oligomeric Intermediates

      • Claire Goldsbury, Janelle Green

      Pages 103-128

   10. Fourier Transform Infrared and Circular Dichroism Spectroscopies for Amyloid Studies

       • Miguel Calero, María Gasset

       Pages 129-151

   11. Quasielastic Light Scattering for Protein Assembly Studies

       • Aleksey Lomakin, David B. Teplow, George B. Benedek

       Pages 153-174

   12. Intrinsic Fluorescent Detection of Tau Conformation and Aggregation

       • Martin von Bergen, Li Li, Eckhard Mandelkow

       Pages 175-184

   13. Quantitative Measurement of Fibrillogenesis by Mass Spectrometry

       • Andrew D. Miranker

       Pages 185-194

3. Cell Culture Assays

   1. Front Matter

      Pages 195-195

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   2. Isolation and Culturing of Human Vascular Smooth Muscle Cells

      • Finnbogi R. Thormodsson, Ingvar H. Olafsson

      Pages 197-210

   3. Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy

      • Sonia S. Jung, Efrat Levy

      Pages 211-219

   4. Purification of Human Wild-Type or Variant Cystatin C From Conditioned Media of Transfected Cells

      • Frances Prelli, Monika Pawlik, Blas Frangione, Efrat Levy

      Pages 221-226

   5. Prion Propagation in Cell Culture

      • Sylvain Lehmann

      Pages 227-234

4. In Vivo-Related Assays

   1. Front Matter

      Pages 235-235

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A proven collection of readily reproducible techniques for studying amyloid proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

• ELISA
• Termination
• Vivo
• biology
• electron microscopy
• molecular biology
• proteins
• reproducible techniques

• Departments of Psychiatry and Pathology, New York University School of Medicine, New York, NY

  Einar M. Sigurdsson

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