Abstract
Gene transfer is a widely used experimental approach to determine the value of specific genes under a variety of conditions. This chapter focuses on the expression of two human antiviral genes, interferon (IFN)-α2 and IFN-β, driven by a cytomegalovirus immediate-early promoter in a human Müller cell line. The anti-viral efficacy of these two transgenes is determined by measuring resistant to herpes simplex virus type 1 infection assessing viral antigen expression and viral titers recovered from control and IFN transgene-transfected cells. Furthermore, comparing the antiviral efficacy of these two transgenes suggest the anti-viral environment elicited by the human IFN-α2 transgene is superior to that of the human IFN-β, which may be related to the amount of biologically active IFN secreted by these cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Adler, H., Beland, J. L., Del-Pan, N. C., Kobzik, L., Sobel, R. A., and Rimm, I. J. (1999) In the absence of T cells, natural killer cells protect from mortality due to, H. S.V-1 encephalitis. J. Neuroimmunol. 93, 208–213.
Tumpey, T. M., Chen, S., Oakes, J. E., and Lausch, R. N. (1996) Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea. J. Virol. 70, 898–904.
Niemialtowski, M. G. and Rouse, B. T. (1992) Predominance of, T.h1 cells in ocular tissues during herpetic stromal keratitis. J. Immunol. 149, 3035–3039.
Liu, T., Tang, Q., and Hendricks, R. L. (1996) Inflammation infiltration of the trigeminal ganglion after herpes simplex virus type 1 corneal infection. J. Virol. 70, 264–271.
Shimeld, C., Efstathiou, S., and Hill, T. (2001) Tracking the spread of a lacZ-tagged herpes simplex virus type 1 between the eye and the nervous system of the mouse: Comparison of primary and recurrent infection. J. Virol. 75, 5252–5262.
Enquist, L. W., Husak, P. J., Banfield, B. W., and Smith, G. A. (1998) Infection and spread of alphaherpesviruses in the nervous system. Adv. Virus Res. 51, 237–347.
Atherton, S. S. and Streilein, W. J. (1987) Two waves of virus following anterior chamber inoculation of, H. S.V-1. Invest. Ophthalmol. Vis. Sci. 28, 571–579.
Azumi, A. and Atherton, S. S. (1994) Sparing of the ipsilateral retina after anterior chamber inoculation of, H. S.V-1: Requirement for either CD4+ or CD8+ T cells. Invest. Ophthalmol. Vis. Sci. 35, 3251–3259.
Tanigawa, M., Bigger, J. E., Kanter, M. Y., and Atherton, S. S. (2000) Natural killer cells prevent direct anterior-to-posterior spread of herpes simplex virus type 1 in the eye. Invest. Ophthalmol. Vis. Sci. 41, 132–137.
Spencer, B., Agarwala, S., Miskulin, M., Smith, M., and Brandt, C. R. (2000) Herpes simplex virus-mediated gene delivery to the rodent system. Invest. Ophthalmol. Vis. Sci. 41, 1392–1401.
Carr, D. J. J, Chodosh, J., Ash, J., and Lane, T. E. (2003) Effect of anti-CXCL10 monoclonal antibody on herpes simplex virus type 1 keratitis and retinal infection. J. Virol. 77, 10037–10046.
Lewis, M. L., Culbertson, W. W., Post, J. D., Miller, D., Kokame, G. T., and Dix, R. D. (1989) Herpes simplex virus type 1. A cause of the acute retinal necrosis syndrome. Ophthalmology 96, 875–878.
Ezra, E., Pearson, R. V., Etchells, D. E., and Gregor, Z. J. (1995) Delayed fellow involvement in acute retinal necrosis syndrome. Am. J. Ophthalmol. 120, 115–116.
Falcon, P. M. and Brockhurst, R. J. (1993) Delayed onset of bilateral acute retinal necrosis syndrome; a 34-year interval. Ann. Ophthalmol. 25, 373–374.
Sarthy, V. and Ripps, H. (2001) The Retinal Müller Cell, 1st ed. New York, Plenum Publishers.
Härle, P., Lauret, E., Pitha, P. M., De, M.aeyer, E., and Carr, D. J. J. (2001) Expression of human and macaque type, I. IFN transgenes interferes with, H. S.V-1 replication at the transcriptional and translational levels: IFN-β is more potent than IFN-α2. Virology 290, 237–248.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
John-Philip, B., Carr, D.J.J. (2005). Transfection of Müller Cells With Type I Interferon Transgenes. In: Carr, D.J.J. (eds) Interferon Methods and Protocols. Methods in Molecular Medicine™, vol 116. Humana Press. https://doi.org/10.1385/1-59259-939-7:221
Download citation
DOI: https://doi.org/10.1385/1-59259-939-7:221
Publisher Name: Humana Press
Print ISBN: 978-1-58829-418-0
Online ISBN: 978-1-59259-939-4
eBook Packages: Springer Protocols