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Detection of Nucleic Acid Interactions Using Surface Plasmon Resonance

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RNA-Protein Interaction Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 118))

Abstract

Several different methods can be used for detection of interactions between RNA and other molecules (e.g., proteins and other nucleic acids). Among these are: (1) enzymatic assays in which there is a measurable change in the property of the substrate such as an increase or decrease in size (mobility in gels or solubility in trichloroacetic acid), (2) gel-shift assays in which the interaction can be detected by altered migration of a complex in gel-electrophoretic analysis, (3) Northwestern assays and Northern analysis in which either proteins or RNA are transferred to a membrane after gel electrophoresis and RNAs are added in solution to interact with the protein or anneal with the RNA on the membrane and others. Each of these techniques has advantages and limitations.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA

    Robert J. Crouch

  2. Department of Research and Development, Kishimoto Clinical Laboratory Group 2-3-9, Hiyoshi-cho, Tomakomai-shi, Hokkaido, Japan

    Makoto Wakasa

  3. Department of Material and Life Sciences, Graduate School of Engineering, Osaka University, Osaka, Japan

    Mitsuru Haruki

Authors
  1. Robert J. Crouch
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  2. Makoto Wakasa
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  3. Mitsuru Haruki
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Editor information

Editors and Affiliations

  1. Uniformed Services University of the Health Sciences, Bethesda, Maryland

    Susan R. Haynes

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© 1999 Humana Press Inc.

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Crouch, R.J., Wakasa, M., Haruki, M. (1999). Detection of Nucleic Acid Interactions Using Surface Plasmon Resonance. In: Haynes, S.R. (eds) RNA-Protein Interaction Protocols. Methods in Molecular Biology™, vol 118. Humana Press. https://doi.org/10.1385/1-59259-676-2:143

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  • DOI: https://doi.org/10.1385/1-59259-676-2:143

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-568-3

  • Online ISBN: 978-1-59259-676-8

  • eBook Packages: Springer Protocols

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