Abstract
The airway epithelium is a target of inflammatory, environmental, and physical stimuli in diseases such as asthma and bronchopulmonary dysplasia. Damage to the epithelium may compromise both the physical barrier and key metabolic functions. Repair involves the migration and spreading of cells over the basement membrane and the proliferation of new epithelial cells. Each step can be modulated actively by growth factors secreted by constitutive cells within the airway, or suppressed by mediators secreted by inflammatory cells that have migrated into the airway. Understanding these steps is essential to gaining insight into the repair process in airway epithelium.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Keenan, K., Combs, J., and McDowell, E. (1982) Regeneration of hamster tracheal epithelium after mechanical injury. I. Focal lesions: quantitative morphologic study of cell proliferation. Virchows Arch. Cell Pathol. 41, 193ā214.
ErjefƤlt, I., ErjefƤlt, J., Persson, C., and Sundler, F. (1995) In vivo restitution of airway epithelium. Cell Tissue Res. 281, 305ā316.
ErjefƤlt, J., Persson, C., and Sundler, F. (1996) Eosinophils, neutrophils, and venular gaps in the airway mucosa at epithelial removal-restitution. Am. J. Respir. Crit. Care Med. 153, 1666ā1674.
Gordon, R. and Lane, B. (1976) Regeneration of rat tracheal epithelium after mechanical injury. II. Restoration of surface integrity during the early hours after injury. Am. Rev. Respir. Dis. 113, 799ā807.
Persson, C., ErjefƤlt, J., ErjefƤlt, I., Korsgren, M., Nilsson, M., and Sundler, F. (1996) Epithelial sheddingārestitution as a causative process in airway inflammation. Clin. Exp. Allergy 26, 746ā755.
Kim, J. S., McKinnis, V. S., and White, S. R. (1997) Proliferation and repair of guinea pig tracheal epithelium after neuropeptide depletion and injury in vivo. Am. J. Physiol. (Lung Cell. Mol. Physiol. 17) 273, L1235āL1241.
Li, X., Dorscheid, D. R., and White, S. R. (2000) Glycosylation profiles of airway epithelial repair after mechanical injury in vivo in guinea pigs. Histochem. J. 32, 207ā216.
Thet, L. A. (1983) A simple method for intubating rats under direct vision. Lab. Anim. Sci. 33, 368ā369.
Costa, D. L., Lehmann, J. R., Harold, W. M., and Drew, R. T. (1986) Transoral tracheal intubation of rodents using a fiberoptic laryngoscope. Lab. Anim. Sci. 36, 256ā261.
Jou, I.-M., Tsai, Y.-T., Tsai, C.-L., Wu, M.-H., Chang, H.-Y., and Wang, N. S. (2000) Simplified rat intubation using a new oropharyngeal intubation wedge. J. Appl. Physiol. 89, 1766ā1770.
Alpert, M., Goldstein, D., and Triner, L. (1982) Technique of endotracheal intubation in rats. Lab. Anim. Sci. 33, 78, 79.
Kuwano, K., Bosken, C. H., Pare, P. D., Bai, T. R., Wiggs, B. R., and Hogg, J. C. (1993) Small airway dimensions in asthma and in chronic obstructive pulmonary disease. Am. Rev. Respir. Dis. 148, 1220ā1225.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
Ā© 2003 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
White, S.R. (2003). Wound Healing in Airways In Vivo. In: DiPietro, L.A., Burns, A.L. (eds) Wound Healing. Methods in Molecular Medicineā¢, vol 78. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-332-1:121
Download citation
DOI: https://doi.org/10.1385/1-59259-332-1:121
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-999-5
Online ISBN: 978-1-59259-332-3
eBook Packages: Springer Protocols