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A Mechanistic Study to Determine the Structural Similarities Between Artificial Membrane Strat-M™ and Biological Membranes and Its Application to Carry Out Skin Permeation Study of Amphotericin B Nanoformulations

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Abstract

Type of biological membrane used in skin permeation experiment significantly affects skin permeation and deposition potential of tested formulations. In this study, a comparative study has been carried out to evaluate the potential of a synthetic membrane (Strat-M™) with rat, human, and porcine ear skin to carry out skin permeation study of nanoformulations of a high molecular weight drug, amphotericin B. Results demonstrated that the permeation of this high molecular weight drug through Strat-M™ showed close similitude to human skin. Value of correlation coefficient (R2) of log diffusion between Strat-M™ and human skin was found to be 0.99 which demonstrated the similarities of Strat-M™ membrane to the human skin. In similarity factor analysis, the value of f2 was also found to be 85, which further demonstrated the similarities of Strat-M™ membrane to human skin. Moreover, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis of synthetic and biological membranes depicted almost similar morphological features (thickness, pore size, surface morphology, and diameter) of synthetic membrane with human skin. The results of the study demonstrated Strat-M™ as a better alternative to carry out skin permeation experiment due to the consistent results, reproducibility, easy availability, and minimum variability with human skin.

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Acknowledgements

One of authors Ms. Lakhvir Kaur is thankful to DST, New Delhi, for providing senior research fellowship under INSPIRE scheme. The authors are thankful to UGC, New Delhi for providing the financial assistance [scheme No. 42-673/2013(SR)]

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab, 143005, India

    Lakhvir Kaur & Subheet Kumar Jain

  2. Department of Microbiology, Government Medical College, Amritsar, 143001, India

    Kanwaldeep Singh

  3. Department of Pathology, Government Medical College, Amritsar, 143001, India

    Surinder Paul

  4. Department of Chemistry, Guru Nanak Dev University, Amritsar, 143005, India

    Sukhprit Singh

  5. Indian Institute of Integrative Medicine, Jammu, 180001, India

    Shashank Singh

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  1. Lakhvir Kaur
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  2. Kanwaldeep Singh
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All authors contributed in design of this manuscript in one or other aspects.

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Correspondence to Subheet Kumar Jain.

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All animal experimentations were performed after approval by the Institutional Animal Ethics Committee of Guru Nanak Dev University, Amritsar (Registration no. 226/PO/Re/S/2000/CPCSEA).

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The authors declare that they have no conflicts of interest.

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Kaur, L., Singh, K., Paul, S. et al. A Mechanistic Study to Determine the Structural Similarities Between Artificial Membrane Strat-M™ and Biological Membranes and Its Application to Carry Out Skin Permeation Study of Amphotericin B Nanoformulations. AAPS PharmSciTech 19, 1606–1624 (2018). https://doi.org/10.1208/s12249-018-0959-6

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