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Comparative Pharmacokinetic Study of Mangiferin After Oral Administration of Pure Mangiferin and US Patented Polyherbal Formulation to Rats

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Abstract

The US patented polyherbal formulation for the prevention and management of type II diabetes and its vascular complications was used for the present study. The xanthone glycoside mangiferin is one of the major effector constituents in the Salacia species with potential anti-diabetic activity. The pharmacokinetic differences of mangiferin following oral administration of pure mangiferin and polyherbal formulation containing Salacia species were studied with approximately the same dose 30 mg/kg mangiferin and its distribution among the major tissue in Wistar rats. Plasma samples were collected at different time points (15, 30, 60, 120, 180, 240, 360, 480, 600, 1,440, 2,160, and 2880 min) and subsequently analyzed using a validated simple and rapid LC-MS method. Plasma concentration versus time profiles were explored by non-compartmental analysis. Mangiferin plasma exposure was significantly increased when administered from formulation compared to the standard mangiferin. Mangiferin resided significantly longer in the body (last mean residence time (MRTlast)) when given in the form of the formulation (3.65 h). Cmax values of formulation (44.16 μg/mL) administration were elevated when compared to equivalent dose of the pure mangiferin (15.23 μg/mL). Tissue distribution study of mangiferin from polyherbal formulation was also studied. In conclusion, the exposure of mangiferin is enhanced after formulation and administration and could result in superior efficacy of polyherbal formulation when compared to an equivalent dose of mangiferin. The results indicate that the reason which delays the elimination of mangiferin and enhances its bioavailability might the interactions of the some other constituents present in the polyherbal formulation. Distribution study results indicate that mangiferin was extensively bound to the various tissues like the small intestine, heart, kidney, spleen, and liver except brain tissue.

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Acknowledgments

The authors would like to thank the Department of Science and Technology, Government of India, for providing the financial assistance to carry the work and Department of Pharmacology, SRM College of Pharmacy, SRM University, for providing facilities.

Conflict of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Interdisciplinary School of Indian System of Medicine (ISISM), SRM University, Kattankulathur, Kancheepuram (Dt), Tamil Nadu, 603203, India

    Ananth Kumar Kammalla, Mohan Kumar Ramasamy, Jyothi Inampudi & Ilango Kaliappan

  2. National Facility for Tribal and Herbal Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

    Govind Prasad Dubey

  3. Faculty of Ayurveda, Institute of Medical sciences, Banaras Hindu University, Varanasi, India

    Aruna Agrawal

Authors
  1. Ananth Kumar Kammalla
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  2. Mohan Kumar Ramasamy
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  3. Jyothi Inampudi
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  4. Govind Prasad Dubey
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  5. Aruna Agrawal
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  6. Ilango Kaliappan
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Corresponding author

Correspondence to Ilango Kaliappan.

Electronic supplementary material

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12249_2014_206_MOESM1_ESM.doc

Practical protocol for isolation of mangiferin was developed in the present study by modifying the previously reported method and its Mass spectrum (Fig 1S) , IR (Fig 2S), 1H and13C NMR spectra (q) of the compound was available as supporting information. (DOC 185 kb)

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Kammalla, A.K., Ramasamy, M.K., Inampudi, J. et al. Comparative Pharmacokinetic Study of Mangiferin After Oral Administration of Pure Mangiferin and US Patented Polyherbal Formulation to Rats. AAPS PharmSciTech 16, 250–258 (2015). https://doi.org/10.1208/s12249-014-0206-8

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  • DOI: https://doi.org/10.1208/s12249-014-0206-8

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