Abstract
The applicability of serological tests in the diagnosis of intrauterine infections in Danish cattle was investigated. Fetuses from slaughter animals, experimentally infected and spontaneously aborted fetuses, premature and stillborn calves, were subjected to necropsy and histological studies, and to microbiological and serological examinations. The latter comprised rocket immunoelectrophoresis and radial immunodiffusion. In sera of the fetuses from slaughter animals, immunoglobulins were either not detectable or only present in very small quantities, whereas sera of fetuses and calves with pathological changes and/or verified infection contained considerable amounts of IgM and IgG. IgA was also detected in the latter group. The results corroborate the diagnostic importance of immunoglobulin determination in aborted fetuses and stillborn calves.
Sammendrag
Anvendeligheden af serologiske tests, som et bidrag til diagnostisering af intrauterine infektioner, blev undersøgt på et dansk kvægmateriale. Fostre fra slagtekvæg, eksperimentelt inficerede og spontant aborterede fostre samt præmature og dødfødte kalve blev underkastet makro- og mikroskopiske, mikrobiologiske og serologiske undersøgelser. Sidstnævnte omfattede raket immunoelektroforese og radial immun diffusion.
Immunoglobulin er var kun påviselige i små mængder hos ganske få fostre fra slagtekvæg, mens sera fra fostre og kalve med patologiske forandringer og/eller verificeret infektion indeholdt betydelige mængder IgM og IgG. IgA kunne også påvises i sera fra denne individgruppe. Resultaterne viser den diagnostiske betydning af en immunoglobulinbestemmelse for aborterede fostre og dødfødte kalve.
Article PDF
Similar content being viewed by others
References
Appleman, R. D. & F. G. Owen: Breeding, housing and feeding management. J. Dairy Sci. 1975, 58, 447–464.
Bellamy, J. E. C. & N. O. Nielsen: Immune-mediated emigration of neutrophils into the lumen of the small intestine. Inf. Immun. 1974, 9, 615–619.
Bellamy, J. E. C. & S. D. Acres: Enterotoxigenic colibacillosis in colostrum-fed calves: Pathologic changes. Amer. J. vet. Res. 1979, 40, 1391–1397.
Doughri, A. M., K. P. Altera, J. Storz & A. K. Eugster: Ultrastructural changes in the chlamydia-infected ileal mucosa of newborn calves. Vet. Path. 1973, 10, 114–123.
Doughri, A. M., S. Young & J. Storz: Pathologic changes in intestinal chlamydial infection of newborn calves. Amer. J. vet. Res. 1974, 35, 939–944.
Doughri, A. M. & J. Storz: Light and ultrastructural pathologic changes in intestinal Coronavirus infection of newborn calves. Zbl. Vet. Med. B. 1977, 24, 367–385.
Husband, A. J., M. R. Brandon & A. K. Lascelles: Absorption and endogenous production of immunoglobulins in calves. In W. A. Hemmings: Antigen Absorption by the Gut. MTP Press Ltd., Lancaster 1978, p. 93–107.
Keenan, K. P., H. R. Jervis, R. H. Marchwicki & L. N. Binn: Intestinal infection of neonatal dogs with canine Coronavirus 1–71: Studies by virologic, histologic, histochemical, and immunofluorescent techniques. Amer. J. vet. Res. 1976, 37, 247–256.
Kent, T. H. & H. W. Moon: The comparative pathogenesis of some enteric diseases. Based on cases presented at the 22nd annual seminar of the American College of Veterinary Pathologists. Vet. Path. 1973, 10, 414–469.
Krunajevic, T.: Experimental virus enteritis in mink. A pathologicanatomical and electron microscopical study. Acta vet. scand. 1970, 11, Suppl. 30.
Laksesvela, B., P. Slagsvold & T. Landsverk: Indigestion in young calves. III. The influence of powders from heat-treated skim-milk and whey. Acta vet. scand. 1978, 19, 159–165.
Landsverk, T.: The gastrointestinal mucosa in young milk-fed calves. A scanning electron and light microscopic investigation. Acta vet. scand. 1979, 20, 572–582.
Landsverk, T.: The epithelium covering Peyer’s patches in young milk-fed calves. An ultrastructural and enzyme histochemical investigation. Acta vet. scand. 1981 a, 22, 198–210.
Landsverk, T.: An enzyme histochemical investigation of the intestinal mucosa in diarrheic calves. Acta vet. scand. 1981 b, 22, 449–458.
Landsverk, T.: Peyer’s patches and the follicle-associated epithelium in diarrheic calves. Pathomorphology, morphometry and acid phosphatase histochemistry. Acta vet. scand. 1981 c, 22, 459– 471.
Landsverk, T. et al.: In prep.
Mebus, C. A., E. L. Stair, N. R. Underdahl & M. J. Twiehaus: Pathology of neonatal calf diarrhea induced by a reo-like virus. Vet. Path. 1971, 8, 490–505.
Mebus, C. A., E. L. Stair, M. B. Rhodes & M. J. Twiehaus: Pathology of neonatal calf diarrhea induced by a coronavirus-like agent. Vet. Path. 1973, 10, 45–64.
Morin, M., P. Lamothe, A. Gagnon & R. Malo: A case of viral neonatal calf diarrhea in a Quebec dairy herd. Canad. J. comp. Med. 1974, 38, 236–242.
Mowen, J. M. V. M.: White Scours in Piglets at Three Weeks of Age. Proefschrift, Rotterdam 1972.
Padykula, H. A.: Recent functional interpretations of intestinal morphology. Fed. Proc. 1962, 21, 873–879.
Pearson, G. R. & E. F. Logan: The rate of development of post mortem artefact in the small intestine of neonatal calves. Brit. J. exp. Path. 1978, 59, 178–182.
Pearson, G. R., M. S. McNulty & E. F. Logan: Pathological changes in the small intestine of neonatal calves with enteric colibacillosis. Vet. Path. 1978, 15, 92–101.
Rijke, R. P. C., W. R. Hanson, H. M. Plaisier & J. W. Osborne: The effect of ischemic villus cell damage on crypt cell proliferation in the small intestine. Evidence for a feedback control mechanism. Gastroenterology 1976, 71, 786–792.
Roy, J. H. B.: Problems in the nutrition of the preruminant calf. Proc. Nutr. Soc. 1974, 33, 79–86.
Specht, W.: Morphology of the intestinal wall. Its mucous membrane under normal and experimental conditions. In M. Kramer and F. Lauterbach: Intestinal Permeation. Proc. 4. Workshop Conf. Schloss Reisenburg, Excerpta Medica, Amsterdam 1977, p. 4–40.
Stair, E. L., C. A. Mebus, M. J. Twiehaus & N. R. Underdahl: Neonatal calf diarrhea. Electron microscopy of intestine infected with a reovirus-like agent. Vet. Path. 1973, 10, 155–170.
Teir, H. & T. Rytomaa: Elimination of granulocytes in the intestinal tract and its pathological consequences. In E. Bajusz and G. Jasmin: Methods and Achievements in Experimental Pathology, vol. 1. S. Karger, Basel and New York 1966, p. 639–676.
Todd, W. J., A. M. Doughri & J. Storz: Ultrastructural changes in host cellular organelles in the course of the chlamydial developmental cycle. Zbl. Bakt. Hyg. I. Abt. Orig. A 1976, 236, 359–373.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohmann, H.B. Immunoglobulin Levels in Non-Aborted and Aborted Fetuses from Danish Herds of Cattle. Acta Vet Scand 22, 428–434 (1981). https://doi.org/10.1186/BF03548668
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03548668