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SRC-3 Plays a Critical Role in Human Umbilical Vein Endothelial Cells by Regulating the PI3K/Akt/mTOR Pathway in Preeclampsia

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Abstract

Preeclampsia (PE) is currently thought to be characterized by oxidative stress which may lead to endothelial dysfunction. The normal function of vascular endothelium is essential to vascular homeostasis. Previous studies have shown that steroid receptor coactivator 3 (SRC-3) interacts with estrogen receptors (ERs) which are involved in the vasoprotective effects of estrogen and is also associated with cell migration, invasion, and inflammation; however, its role in PE remains unclear. The main purpose of this study is to identify the role of SRC-3 in the function of human umbilical vein endothelial cells (HUVECs) during the development of PE. Our study demonstrated that the expression of SRC-3 was significantly decreased in PE placentas compared to normal placentas. Additionally, lentivirus short hairpin RNA against SRC-3 and hypoxia/reoxygenation treatments attenuated migration and tube formation abilities and enhanced HUVEC apoptosis. Furthermore, we detected possible downstream in the PI3K/Akt/ mammalian target of rapamycin (mTOR) signal pathway activity, which is involved in SRC-3-mediated HUVEC function. Our data suggest that oxidative stress plays a crucial role in controlling SRC-3 expression, which influences the migration and tube formation abilities of endothelial cells through the PI3K/Akt/mTOR signaling pathways. This action may then result in PE pathogenesis.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, No. I Youyi Road, Yuzhong District, Chongqing, China

    Yu Yuan MD, Nan Shan MD, Bin Tan MD, Qinyin Deng MD, Yangming Liu MD, Hanbin Wang MD, Xiaofang Luo MD, Chengjin He MD, Xin Luo MD, Hua Zhang MD & Hongbo Qi MD

  2. China-Canada-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, China

    Yu Yuan MD, Nan Shan MD, Bin Tan MD, Qinyin Deng MD, Yangming Liu MD, Hanbin Wang MD, Xiaofang Luo MD, Chengjin He MD, Xin Luo MD, Hua Zhang MD, Philip N. Baker MD, David M. Olson MD & Hongbo Qi MD

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  1. Yu Yuan MD
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Correspondence to Hongbo Qi MD.

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Yuan, Y., Shan, N., Tan, B. et al. SRC-3 Plays a Critical Role in Human Umbilical Vein Endothelial Cells by Regulating the PI3K/Akt/mTOR Pathway in Preeclampsia. Reprod. Sci. 25, 748–758 (2018). https://doi.org/10.1177/1933719117725818

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