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Periostin Enhances Migration, Invasion, and Adhesion of Human Endometrial Stromal Cells Through Integrin-Linked Kinase 1/Akt Signaling Pathway

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Abstract

Although our previous study confirmed that periostin (PN) was overexpressed in the eutopic and ectopic endometrial stroma of women with endometriosis by immunohistochemitry, the role of PN in the pathophysiology of endometriosis remains unknown. Thus, we aimed to investigate the effects of PN on endometrial stromal cells (ESCs) migration, invasion, adhesion, and proliferation and to further study the mechanism under this process. Eutopic (EuSCs), ectopic (EcSCs), and normal ESCs (NSCs) were isolated and cultured. We evaluated the above-mentioned biology behaviors and the expression of PN, integrin-linked kinase 1 (ILK1), and phospho-Akt (p-Akt) in NSCs, EuSCs as well as EcSCs before and after receiving PN small-interfering RNA (siRNA). The protein and messenger RNA (mRNA) levels of PN were upregulated in EuSCs (P < .05; P = .2261 in proliferative phase and P = .3385 in secretory phase) and EcSCs (P < .001; P < .001 in proliferative phase and P < .05 in secretory phase) compared with NSCs, although there was no significant difference in PN mRNA between EuSCs and NSCs. In EcSCs, abilities of migration, invasion, and adhesion and the expressions of ILK1 and p-Akt were enhanced; and all of those were downregulated after PN siRNA interference. Thus, PN enhanced ESCs migration, invasion, and adhesion due to the ILK1/Akt signal pathway. As an agonist in the development and progression of endometriosis, PN may be a new clinical treatment target of endometriosis.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, No. 107 Wenhua Xi Road, Lixia District, Jinan, Shandong, China

    Xiaoxuan Xu MD, Qiaomei Zheng PhD, Xiaolei Zhang PhD, Ruihan Liu MD & Peishu Liu PhD

  2. Department of Orthopedics, Taishan Medical University, Tai’an, Shandong, China

    Zongzheng Zhang MD

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  1. Xiaoxuan Xu MD
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  2. Qiaomei Zheng PhD
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  3. Zongzheng Zhang MD
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  4. Xiaolei Zhang PhD
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  5. Ruihan Liu MD
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  6. Peishu Liu PhD
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Correspondence to Peishu Liu PhD.

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Xu, X., Zheng, Q., Zhang, Z. et al. Periostin Enhances Migration, Invasion, and Adhesion of Human Endometrial Stromal Cells Through Integrin-Linked Kinase 1/Akt Signaling Pathway. Reprod. Sci. 22, 1098–1106 (2015). https://doi.org/10.1177/1933719115572481

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