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Glucose and Fatty Acid Metabolism in Placental Explants From Pregnancies Complicated With Gestational Diabetes Mellitus

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Abstract

Placental metabolism is an important mechanism for the regulation of fetal growth and long-term health of the newborns. In this study, we investigated the effects of maternal metabolic environment on human placental fatty acid and glucose metabolism. We used placental explants from uncomplicated pregnancies or pregnancies complicated with gestational diabetes mellitus (GDM), undergoing vaginal delivery (VD) or cesarean section (CS). Fatty acid oxidation (FAO) and glucose uptake (2-DOG) were similar in both modes of delivery in normal and GDM pregnancies. However, placental explants from GDM exhibited 40% to 50% reduced FAO capacity compared to control placentas in women undergoing VD or CS. In contrast, 2-DOG uptake was 2- to 3-fold higher in placental explants from GDM compared to control placentas in women undergoing VD or CS, respectively. In conclusion, ex vivo placental fuel selection is influenced by maternal GDM, but placental metabolic characteristics are not altered by the mode of delivery.

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References

  1. Barbour LA. Unresolved controversies in gestational diabetes: implications on maternal and infant health. Curr Opin Endocrinol Diabetes Obes. 2014;21(4):264–270.

    CAS  PubMed  Google Scholar 

  2. Catalano PM, Hauguel-De Mouzon S. Is it time to revisit the Pedersen hypothesis in the face of the obesity epidemic? Am J Obstet Gynecol. 2011; 204(6):479–487.

    PubMed  PubMed Central  Google Scholar 

  3. Burton GJ, Sebire NJ, Myatt L, et al. Optimising sample collection for placental research. Placenta. 2014;35(1):9–22.

    CAS  PubMed  Google Scholar 

  4. Mendez-Figueroa H, Chien EK, Ji H, Nesbitt NL, Bharathi SS, Goetzman E. Effects of labor on placental fatty acid beta oxidation. J Matern Fetal Neonatal Med. 2012;26(2):150–154.

    PubMed  PubMed Central  Google Scholar 

  5. Visiedo F, Bugatto F, Sanchez V, Cozar-Castellano I, Bartha JL, Perdomo G. High glucose levels reduce fatty acid oxidation and increase triglyceride accumulation in human placenta. Am J Physiol Endocrinol Metab. 2013; 305(2):E205–E212.

    CAS  PubMed  Google Scholar 

  6. Brar HS, Platt LD, DeVore GR, Horenstein J, Medearis AL. Qualitative assessment of maternal uterine and fetal umbilical artery blood flow and resistance in laboring patients by Doppler velocimetry. Am J Obstet Gynecol. 1988;158(4):952–956.

    CAS  PubMed  Google Scholar 

  7. Fleischer A, Anyaegbunam AA, Schulman H, Farmakides G, Randolph G. Uterine and umbilical artery velocimetry during normal labor. Am J Obstet Gynecol. 1987;157(1):40–43.

    CAS  PubMed  Google Scholar 

  8. Bartha JL, Visiedo F, Fernandez-Deudero A, Bugatto F, Perdomo G. Decreased mitochondrial fatty acid oxidation in placentas from women with preeclampsia. Placenta. 2012;33(2): 132–134.

    CAS  PubMed  Google Scholar 

  9. Perdomo G, Martinez-Brocca MA, Bhatt BA, Brown NF, O’Doherty RM, Garcia-Ocana A. Hepatocyte growth factor is a novel stimulator of glucose uptake and metabolism in skeletal muscle cells. J Biol Chem. 2008;283(20):13700–13706.

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Yung HW, Colleoni F, Atkinson D, et al. Influence of speed of sample processing on placental energetics and signalling pathways: implications for tissue collection. Placenta. 2014;35(2):103–108.

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Di Santo S, Malek A, Sager R, Andres AC, Schneider H. Trophoblast viability in perfused term placental tissue and explant cultures limited to 7–24 hours. Placenta. 2003; 24(8–9):882–94.

    PubMed  Google Scholar 

  12. Baumann MU, Schneider H, Malek A, et al. Regulation of human trophoblast GLUT1 glucose transporter by insulin-like growth factor I (IGF-I). PLoS One. 2014; 9(8):e106037.

    PubMed  PubMed Central  Google Scholar 

  13. Ramsay TG, Karousis J, White ME, Wol verton CK. Fatty acid metabolism by the porcine placenta. J Anim Sci. 1991;69(9):3645–3654.

    CAS  PubMed  Google Scholar 

  14. Jansson T, Wennergren M, Powell TL. Placental glucose transport and GLUT 1 expression in insulin-dependent diabetes. Am J Obstet Gynecol. 1999;180(1 pt 1):163–168.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Research Unit, Puerta del Mar University Hospital, Cádiz, Spain

    Francisco Visiedo PhD, Irene Cózar-Castellano PhD & Germán Perdomo PhD

  2. Department of Obstetrics and Gynecology, Puerta del Mar University Hospital, Cádiz, Spain

    Fernando Bugatto PhD, MD & Jose L. Bartha PhD, MD

  3. Division of Maternal-Fetal Medicine, Ginemed, Sevilla, Spain

    Rocío Quintero-Prado MD

  4. Instituto de Genética y Biología Molecular (IBGM)-Universidad de Valladolid, Valladolid, Spain

    Irene Cózar-Castellano PhD

  5. Department of Obstetrics and Gynecology, La Paz University Hospital, Madrid, Spain

    Jose L. Bartha PhD, MD

  6. University of Castilla-La Mancha, School of Environmental Sciences and Biochemistry, Science-Technology campus in the old Weapons Factory, Sabatini Building, Avenue of Charles III, s/n, 45071, Toledo, Spain

    Germán Perdomo PhD

Authors
  1. Francisco Visiedo PhD
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  2. Fernando Bugatto PhD, MD
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  3. Rocío Quintero-Prado MD
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  4. Irene Cózar-Castellano PhD
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  5. Jose L. Bartha PhD, MD
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  6. Germán Perdomo PhD
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Corresponding author

Correspondence to Germán Perdomo PhD.

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Visiedo, F., Bugatto, F., Quintero-Prado, R. et al. Glucose and Fatty Acid Metabolism in Placental Explants From Pregnancies Complicated With Gestational Diabetes Mellitus. Reprod. Sci. 22, 798–801 (2015). https://doi.org/10.1177/1933719114561558

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  • DOI: https://doi.org/10.1177/1933719114561558

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