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Expression of Notch Family Proteins in Placentas From Patients With Early-Onset Severe Preeclampsia

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Abstract

Objectives

This study is aimed to identify the expression of Notch family proteins in placentas from patients with early-onset severe preeclampsia.

Study Design

The expression of Notch family proteins in placentas was investigated by immunohistochemistry, Western blotting, and real-time reverse transcription–polymerase chain reaction (RT-PCR).

Results

The profile of distribution of all Notch family proteins in placentas from patients with early-onset severe preeclampsia is similar to that in normal placentas. All Notch family proteins are expressed in placental trophoblasts. Moreover, Notch1 and Jagged1 (Jag1) are detected in placental endothelial cells. Real-time RT-PCR showed that messenger RNA levels of Notch2 and Delta-like4 (Dll4) in placentas from patients with early-onset severe preeclampsia are lower than that of normal placentas. Western blotting showed a significant increase in Notch3 expression and a significant decrease in Notch2 expression in placentas from patients with early-onset severe preeclampsia relative to those in normal placentas.

Conclusion

The results suggest that Notch2 and Notch3 may play some roles in the pathogenesis of preeclampsia.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P.R. China

    Wei-Xiu Zhao MS, Tao-Tao Huang MS, Meng Jiang MS, Ran Feng BS & Jian-Hua Lin PhD

Authors
  1. Wei-Xiu Zhao MS
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  2. Tao-Tao Huang MS
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  3. Meng Jiang MS
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  4. Ran Feng BS
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  5. Jian-Hua Lin PhD
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Correspondence to Jian-Hua Lin PhD.

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Zhao, WX., Huang, TT., Jiang, M. et al. Expression of Notch Family Proteins in Placentas From Patients With Early-Onset Severe Preeclampsia. Reprod. Sci. 21, 716–723 (2014). https://doi.org/10.1177/1933719113512530

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  • DOI: https://doi.org/10.1177/1933719113512530

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