Abstract
In this study, a bovine dentin, from a material collection which was excavated in Nevşehir–Ürgüp, Turkey, was used. This dentin sample was prepared in the laboratory using cutting, cleaning, grinding, and separating processes, respectively. The obtained dentin aliquots were irradiated with multiply radiation doses between 50 Gy and 2 kGy by 60Co-gamma irradiator. The dosimetric properties and the radiation dose of this sample were determined using the electron paramagnetic resonance (EPR) method. During the measurements, the EPR spectra were recorded at four different temperatures (4 K, 50 K, 100 K, and 298 K) using X-Band ESR spectrometer. By these spectra, it was determined that the radiation-induced paramagnetic radical was occurred, and the accumulated radiation dose was calculated to be (12.404 ± 0.48) Gy. Also, this sample was analyzed in terms of microstructure and porosity using the field emission scanning electron microscopy, elemental composition using the energy-dispersive X-ray spectrometry, mineralogical composition using the X-ray diffraction, and chemical bonding using the Fourier transform infrared spectroscopy. As a result of all these measurements, it was understood that this sample had a single-phase hydroxyapatite crystal.
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05 June 2023
A Correction to this paper has been published: https://doi.org/10.1140/epjp/s13360-023-04134-7
References
T. De, A. Romanyukha, F. Trompier, B. Pass, P. Misra, Feasibility of Q-band EPR dosimetry in biopsy samples of dental enamel, dentine and bone. Appl. Magn. Reson. 44, 375–387 (2013)
N. Nakamura, M. Iwasaki, C. Miyazawa, M. Akiyama, A.A. Awa, Assessing radiation dose recorded in tooth enamel. Rerf 6(2), 6–7 (1994)
K. Usta, Y. Ceylan, A. Usta, N. Ceylan, E. Aras, An EPR study on radiation-ınduced 2-(piperidin-1-ylmethyl) phenol single crystal. Acta Phys. Pol., A 130(81), 178–180 (2016)
O. Karatas, E. Aras, Electron paramagnetic resonance of gamma-irradiated single crystals of ethan-1, 2 disulfonic acid disodium. J. Mol. Struct. 1027, 49–52 (2012)
O. Karatas, E. Aras, A.H. Karadag, Y. Islek, Electron paramagnetic resonance study of gamma (γ)-irradiated methyl 4-methyl benzoate (C9H10O2). Radiat. Effects Defects Solids 171(7–8), 651–657 (2016)
R. Grün, M. Aubert, J. Hellstrom, M. Duval, The challenge of direct dating old human fossils. Quat. Int. 223–224, 87–93 (2010)
M. Duval, R. Grün, J.M. Pares, L. Martin-Frances, I. Campana, J. Rosell, Q. Shao, J.J. Arsuaga, E. Carbonell, J.M.B.D. Castro, The first direct ESR dating of a hominin tooth from Atapuerca Gran Dolina TD-6 (Spain) supports the antiquity of Homo antecessor. Quat. Geochronol. 47, 120–137 (2018)
T. Oka, A. Takahashi, K. Koarai, Y. Mitsuyasu, Y. Kino, T. Sekine, Y. Shimizu, M. Chiba, T. Suzukii, K. Osaka, K. Sasaki, Y. Urushihara, S. Endo, M. Suzuki, H. Shinoda, M. Fukumoto, External exposure dose estimation by electron spin resonance technique for wild Japanese macaque captured in Fukushima Prefecture. Radiat. Meas. 134, 106315 (2020)
A. Todaka, S. Toyoda, M. Natsuhori, K. Okada, I. Sato, H. Sato, J. Saski, ESR assessment of tooth enamel dose from cattle bred in areas. Radiat. Meas. 136, 106357 (2020)
L. Buchbinder, H. Datz, N. Dayan, R. Carmieli, A. Blank, The potential of pulsed electron spin resonance for tooth based retrospective biodosimetry. Appl. Magn. Reson. (2021). https://doi.org/10.1007/s00723-021-01417-z
S. Toyoda, M. Murahashi, M. Natsuhori, S. Ito, A. Ivannikov, A. Todaka, Retrospective ESR reconstruction of cattle tooth enamel doses from the radioactive nuclei released by the accident of Fukushima Dai-Ichi atomic power plants. Radiat. Prot. Dosim. 186(1), 48–53 (2019)
K. Shiraishi, M. Iwasaki, C. Miyazawa, H. Yonehara, M. Matsumoto, Dose estimation by ESR on tooth enamel from two workers exposed to radiation due to the JCO accident. J. Radiat. Res. 43, 331–335 (2002)
R.D.C. Ribeiro, A. Kinoshita, H.I.D. Araujo-Junior, A.N.G. Figueiredo, I.D.S. Carvalho, ESR dating of Toxodon teeth from Baixa Grande, Bahia, Brazil. J. South Am. Earth Sci. 112(2), 103616 (2021)
A.R. Skinner, B.A.B. Blackwell, J.I.B. Blickstein, J. Lundberg, Using dentine as well as enamel in ESR dating. Braz. J. Phys. 52(1), 25 (2022)
G. Shani, Radiaiton dosimetry, 2nd edn. (CRC Press LLC, USA, 2001), p.295
R. Grün, Electron spin resonance (ESR) dating. Quatern. Int. 1, 65–109 (1989)
M. Ikeya, New applications of ESR-dating, dosimetry and microscopy, 2nd edn. (World Scientific Publication, Singapore, 1993), p.96
W.J. Rink, Electron spin resonance (ESR) dating and ESR applications in quaternary science and archaeometry. Radiat. Meas. 27, 975–1025 (1997)
Z.M. Da Costa, W.M. Pontuschka, L.L. Campos, Study of the ESR signal of gamma irradiated hydroxyapatite for dose assessment. Nucl. Inst. Methods Phys. Res. B 218, 283–288 (2004)
R. Grün, Direct dating of human fossils. Yearb. Phys. Anthropol. 49, 2–48 (2006)
P. Fattibene, F. Callens, EPR dosimetry with tooth enamel: a review. Appl. Radiat. Isot. 68(11), 2033–2116 (2010)
W.J. Rink, J.W. Thompson, Encyclopedia of scientific dating methods (Springer Publication, Netherlands, 2015), p.240
M. Richard, C. Falgueres, E. Pons-Branchu, L. Foliot, P.M. Guillem, R. Martinez-Valle, A. Eixea, V. Villaverde, “ESR/U-series chronology of early Neanderthal occupations at Cova Negra (Valencia, Spain). Quat. Geochronol. 49, 283–290 (2019)
Baffa, O., Kinoshita, A., Abrego, F.C., Silva, N.A., ESR and NMR dosimetry, Epr in the 21st Century: Basics and Applications to Material, Life and Earth Sciences, 614–623, (2002)
S. Hillson, Teeth, Cambridge manuals in archaeology (Cambridge University Press, 2005)
C. Kendall, A.M. Høier Eriksen, I. Kontopoulosd, M.J. Collins, G. Turner-Walker, Diagenesis of archaeological bone and tooth. Palaeogeogr. Palaeoclimatol. Palaeoecol. 491, 21–37 (2018)
IAEA, (IAEA-TECDOC-1331), Use of electron paramagnetic resonance dosimetry with tooth enamel for retrospective dose assessment, Austria, (2002)
N. Mlakar, Z. Pavlica, M. Petelin, J. Strancar, P. Zrimsek, A. Pavlic, Animal and human dentin microstructure and elemental composition. Cent. Eur. J. Med. 9(3), 468–476 (2014)
R.L. Azevedo, V.K. Asfora, D.S. Mützenberg, D. Cisneiros, H.L. Sullasi, A.M. Kinoshita, P.L. Guzzo, A.R. Skinner, O. Baffa, A.M. Pessis, H.J. Khoury, ESR dating of megafauna enamel teeth from Lagoa Uri de Cima archaeological site (Pernambuco, Northeastern Brazil). Quatern. Int. 556, 38–48 (2020)
L. Bachmann, R. Diebolder, R. Hibst, D.M. Zazell, Infrared absorption bands of enamel and dentin tissues from human and bovine teeth. Appl. Spectrosc. Rev. 38(1), 1–14 (2003)
A. Nanci, Ten Cate’s oral histology: development, structure and function, 6th edn. (Mosby, St. Louis, 2003)
E.H. Haskell, G.H. Kenner, R.B. Hayes, Electron-paramagnetic-resonance dosimetry of dentin following removal of organic material. Health Phys. 68(4), 579–584 (1995)
E.I. Tolstykh, E.A. Shishkina, M.O. Degteva, D.V. Ivanov, V.A. Shved, S.N. Bayankin, L.R. Anspaugh, B.A. Napier, A. Wiese, P. Jacob, Complex experimental research on internal tooth dosimetry for the Techa river region: a model For 90sr accumulation in human teeth formed by time of intakes. Health Phys. 85(4), 409–419 (2003)
P. Fattibene, A. Carosi, V. De Coste, S. Onori, EPR properties of intact and Deproteinated dentin. Radiat. Prot. Dosimetry. 120(1–4), 216–220 (2006)
B. Pass, J.E. Aldrich, Enamel biopsy and tooth restoration for measurements of radiation exposure from nuclear accidents using ESR. J. Dent. Res. 69, 345 (1990)
H.P. Schwarcz, Dating bones and teeth: the beautiful and the dangerous, in Humanity from African Naissance to Coming Millennia. ed. by P.V. Tobia, M.A. Raath, J. Moggi-Cecci, G.A. Doyle (Firenze University Press, Florence, 2000), pp.249–256
M.J. Kohn, Models of diffusion-limited uptake of trace elements in fossils and rates of fossilization. Geochim. Cosmochim. Acta 72(15), 3758–3770 (2008)
A. Ferdianto, Dental preparation process for the electron spinning resonance/U-series dating method. J. Penelit. dan Pengemb. Arkeol 7(2), 167–174 (2018)
Y. Wang, T.E. Cerling, A model of tooth and bone diagenesis: implications for paleodiet reconstruction from stable isotopes. Paleogeogr. Paleoclimatol. Paleoecol. 107, 281–289 (1994)
M.J. Kohn, M.J. Schoeninger, W.W. Barker, Altered states: effects of diagenesis on fossil tooth chemistry. Geochim. Cosmochim. Acta 63, 2737–2747 (1999)
A. Zazzo, C. Lecuyer, A. Mariotti, Experimentally-controlled carbon and oxygen isotope exchange between bioapatites and water under inorganic and microbially-mediated conditions. Geochim. Cosmochim. Acta 68, 1–12 (2004)
J.D. Pasteris, D.Y. Ding, Experimental fluoridation of nanocrystalline apatite. Am. Miner. 94, 53–63 (2009)
C.-C. Wu, S.-T. Huang, T.W. Tsen, Q.-L. Rao, H.C. Lin, FT-IR and XRD investigations on sintered fluoridated hydroxyapatite composites. J. Mol. Struct. 979, 72–76 (2010)
H. Hanlie, T. Liyun, J. Tao, The crystal characteristics of enamel and dentin by XRD method. J. Wuhan Univ. Technol. Mater. Sci. Ed. 21(9), 453 (2006)
R.Z. LeGeros, Calcium phosphates in oral biology and medicine, in Monographs in oral sciences. ed. by H.M. Myers (Karger in Basel, New York, 1991)
S.V. Dorozhkin, M. Epple, Biological and medical significance of calcium phosphates. Angew. Chem. Int. Ed. 41(17), 3130–3146 (2002)
C. Loch, L. Hemm, B. Taylor, I.N. Visser, O. Wiig, Microstructure, elemental composition and mechanical properties of enamel and dentine in the polar bear Ursus maritimus. Arch. Oral Biol. 134, 105318 (2022)
H.B. Lu, C.T. Campbell, D.J. Graham, B.D. Ratner, Surface characterization of hydroxyapatite and related calcium phosphates by XPS and TOF-SIMS. Anal. Chem. 72, 288e694 (2000)
J. Kudkuli, R. Abdulla, P.D. Rekha, S.D. Sharma, O. Gurjar, Spectroscopic analyses reveal radiotherapy-induced variations in elemental composition and crystallite properties of human permanent teeth enamel. J. Oral Biosci. 61, 207e214 (2019)
V.C. Farmer, The infrared spectra of minerals (The Mineralogical Society, London, 1974), p.539
C. Rey, B. Collins, T. Goehl, I.R. Dickson, M.J. Glimcher, The carbonate environment in bone mineral: a resolution enhanced Fourier tranform spectroscopy study. Calcif. Tissue Int. 45, 157–164 (1989)
C. Babot-Marquillas, M.J. Sanchez-Martin, J.M. Amigo, I. Yousef, I.H. Valido, R. Boada, M. Valiente, Tooth whitening, oxidation or reduction? Study of physicochemical alterations in bovine enamel using synchrotron based Micro-FTIR. Dent. Mater. 38, 670–679 (2022)
C.C.A. Lopes, P.H.J.O. Limirio, V.R. Novais, P. Dechichi, Fourier transform infrared spectroscopy (FTIR) application chemical characterization of enamel, dentin and bone. Appl. Spectrosc. Rev. 53, 747–769 (2018)
Gross, K., Yang, T.C., Kareiva. A., Curr. Biol. 24, 503–506, (2014)
D. Rokaya, V. Srimaneepong, J. Sapkota, J. Qin, K. Siraleartmukul, V. Siriwongrungson, Polymeric materials and films in dentistry: an overview. J. Adv. Res. 14, 25–34 (2018)
K.H. Chen, W.T. Cheng, M.J. Li, D.M. Yang, S.Y. Lin, Calcification of senile cataractous lens determined by Fourier transform infrared (FTIR) and Raman microspectroscopies. J. Microsc. 219(36–41), 2005 (2005)
F. Callens, G. Vanhaelewyn, P. Matthys, E. Boesman, EPR of carbonate derived radicals: applications in dosimetry, dating and detection of irradiated food. Appl. Magn. Reson. 14, 235–254 (1998)
R. Grün, Methods of dose determination using ESR spectra of tooth enamel. Radiat. Meas. 32, 767–772 (2000)
R. Grün, P. DeCanniere, ESR dating: problems in the evaluation of the naturally accumulated dose (AD) in secondary carbonates. J. Radioanal. Nucl. Chem. Lett. 85, 213–226 (1984)
M. Barabas, The nature of the paramagnetic centres at g = 2.0057 and g = 2.0031 in marine carbonates. Int. J. Radial. Appl. Instrum. Part D 20(3), 453–464 (1992)
A.O. Acar, M. Polat, T. Aydin, C. Aydaş, The ESR dosimetric features of strontium sulfate and temperature effects on radiation-induced signals. Radiat. Phys. Chem. 123, 31–36 (2016)
A.I. Ivannikov, F. Trompier, E. Gaillard-Lecanu, V.G. Skvortsov, V.F. Stepanenko, Optimisation of recording conditions for the electron paramagnetic resonance signal used in dental enamel dosimetry. Radiat. Prot. Dosim. 101(1–4), 531–538 (2002)
R. Grün, R. Joannes-Boyau, Stringer, “Two types of CO2- radicals threaten the fundamentals of ESR dating of tooth enamel. Quat. Geochronol. 3, 150–172 (2008)
V.E. Galtsev, O.Y. Grinberg, Y.S. Lebedev, E.V. Galtseva, EPR dosimetry sensitivity enhancement by detection of rapid passage signal of the tooth enamel at low temperature. Appl Magn Reson 4, 331–333 (1993)
M. Polat, M. Korkmaz, Effect of radiation on solid paracetamol: ESR identification and dosimetric features of γ-irradiated paracetamol. Radiat. Effects Defects Solids 161, 51–62 (2006)
B. Engin, C. Aydaş, M. Polat, Detection of gamma irradiated fig seeds by analyzing electron spin resonance. Food Chem. 126, 1877–1882 (2011)
A. Kinoshita, E. Mayer, V.R. Mendes, A.M.G. Figueiredo, O. Baffa, Electron spin resonance dating of megafauna from Lagoa dos Porcos, Piauí, Brazil. Radiat. Protect. Dosim. 159, 212–219 (2014)
M. Duval, R. Grün, Are published ESR dose assessments on fossil tooth enamel reliable? Quat. Geochronol. 31, 19–27 (2016)
Acknowledgements
The author acknowledges the partially financial supported by the Selcuk University BAP Coordinatorship with the project number 20401116. I am grateful to Prof. Dr. O. Basoglu for providing the sample and Dr. V. Kataev of Leibniz Institute for Solid State and Materials Research, Dresden, Germany, for good opportunities to work with the X-band ESR spectrometer.
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Karataş, Ö. An experimental study on dosimetric and characteristic properties of bovine dentin. Eur. Phys. J. Plus 138, 24 (2023). https://doi.org/10.1140/epjp/s13360-023-03663-5
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DOI: https://doi.org/10.1140/epjp/s13360-023-03663-5