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Epidermal growth factor promotes a mesenchymal over an amoeboid motility of MDA-MB-231 cells embedded within a 3D collagen matrix

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Abstract.

The receptor of epidermal growth factor (EGFR) critically regulates tumor cell invasion and is a potent therapeutic target for treatment of many types of cancers, including carcinomas and glioblastomas. It is known that EGF regulates cell motility when tumor cells are embedded within a 3D biomatrix. However, roles of EGF in modulating tumor cell motility phenotype are largely unknown. In this article, we report that EGF promotes a mesenchymal over an amoeboid motility phenotype using a malignant breast tumor cell line, MDA-MB-231, embedded within a 3D collagen matrix. Amoeboid cells are rounded in shape, while mesenchymal cells are elongated, and their migrations are governed by a distinctly different set of biomolecules. Using single cell tracking analysis, we also show that EGF promotes cell dissemination through a significant increase in cell persistence along with a moderate increase of speed. The increase of persistence is correlated with the increase of the percentage of the mesenchymal cells within the population. Our work reveals a novel role of microenvironmental cue, EGF, in modulating heterogeneity and plasticity of tumor cell motility phenotype. In addition, it suggests a potential visual cue for diagnosing invasive states of breast cancer cells. This work can be easily extended beyond breast cancer cells.

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Authors and Affiliations

  1. Department of Biological and Environmental Engineering, Cornell University, 14853, Ithaca, NY, USA

    Dongil T. Geum, Beum Jun Kim, Matthew S. Hall & Mingming Wu

  2. Research Apprenticeship in Biological Sciences, Cornell University, 14853, Ithaca, NY, USA

    Audrey E. Chang

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  1. Dongil T. Geum
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  2. Beum Jun Kim
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  4. Matthew S. Hall
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  5. Mingming Wu
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Correspondence to Mingming Wu.

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Geum, D., Kim, B., Chang, A. et al. Epidermal growth factor promotes a mesenchymal over an amoeboid motility of MDA-MB-231 cells embedded within a 3D collagen matrix. Eur. Phys. J. Plus 131, 8 (2016). https://doi.org/10.1140/epjp/i2016-16008-8

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