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Motility and cell shape roles in the rheology of growing bacteria cultures

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Abstract.

Cell shape, size and self-motility appear as determinant intrinsic cell factors in the rheological behavior of living bacterial cultures during the growth process. In this work three different species were considered due to their differences on these intrinsic characteristics: two different strains of Staphylococcus aureus - strain COL and its isogenic cell wall autolysis mutant, RUSAL9 - both non-motile and Escherichia coli and Bacillus subtilis - both presenting intrinsic motility. In situ real-time rheology, was used to characterize the activity of growing bacteria, under steady-shear conditions, in particular the viscosity growth curve was measured, for a constant shear flow rate, presenting for all studied cultures, different and rich flow curves. These complex rheological behaviors are a consequence of two coupled effects: the cell density continuous increase and its changing interacting properties, where cell size and shape and intrinsic motility are major players.

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Authors and Affiliations

  1. Laboratory of Molecular Microbiology of Bacterial Pathogens, UCIBIO@REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal

    R. Portela & R. G. Sobral

  2. Área Departamental de Física, ISEL - Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, Rua Conselheiro Emídio Navarro 1, P-1959-007, Lisboa, Portugal

    P. L. Almeida & C. R. Leal

  3. CENIMAT/I3N, Departamento de Ciência dos Materiais, Faculdade Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal

    P. L. Almeida & C. R. Leal

Authors
  1. R. Portela
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  2. P. L. Almeida
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  3. R. G. Sobral
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  4. C. R. Leal
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Correspondence to C. R. Leal.

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Portela, R., Almeida, P.L., Sobral, R.G. et al. Motility and cell shape roles in the rheology of growing bacteria cultures. Eur. Phys. J. E 42, 26 (2019). https://doi.org/10.1140/epje/i2019-11787-9

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