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Liposomes Containing Esters of the Natural Antioxidant Astaxanthin Modified with Pluronic F68 or DSPE-PEG 2000

  • NANOBIOMEDICINE AND NANOPHARMACEUTICALS
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Abstract

Liposomes containing a natural antioxidant, astaxanthin esters, are prepared by dispersing the lipid film and evaporating from chloroform with further ultrasonic treatment. To increase the stability of the astaxanthin esters, liposomes based on Lipoid S75 (2 mg/mL) are modified with pluronic F68 or PEGylated distearylphosphatidylethanolamine (DSPE-PEG 2000). As a result of optimization, the ratio of phospholipid and modifiers is selected for stable liposomes with 0.5 mg/mL astaxanthin esters. Liposomes with 0.5% pluronic F68 consists of two fractions with sizes of 110 ± 15 and 440 ± 15 nm, and liposomes with DSPE-PEG 2000 (2.5 mg/mL) have one fraction with an average hydrodynamic diameter of 255 ± 40 nm. The ζ potentials of the liposomes are –35 ± 15 and –60 ± 10 mV, respectively. When mononuclear blood cells are incubated with the developed liposomes for 24 h, the survival rate is found to be 84 ± 4%. It is shown that liposomes with astaxanthin esters of different compositions inactivate the ABTS cation radical 17–50% more effectively than liposomes without astaxanthin esters.

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ACKNOWLEDGMENTS

We express our gratitude to the representative office of Lipoid AG and its head A.V. Symon for providing the lipids. The equipment of the “Probe and Electron Microscopy” resource center of the National Research Center “Kurchatov Institute” was used in the work.

Funding

The work was carried out within the framework of the National Research Center “Kurchatov Institute” thematic plan 1f.4.1: “The Study of the processes of Generation, Transmission, and Distribution of Energy in Living Organisms, Aimed at Finding New Approaches to the Creation of Therapeutic Agents, New Bioenergy Devices, and Artificial Photosynthesis Systems” and agreement no. 07-1-21 of Moscow State University “Physical–chemical Properties of Biomembranes under Normal Conditions, in Pathology, and under the Influence of Environmental Factors.”

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Authors and Affiliations

  1. Mendeleev Russian Chemical-Technological University, Moscow, Russia

    N. S. Marchenkova & K. E. Barkar

  2. National Research Center “Kurchatov Institute” , Moscow, Russia

    E. A. Kulikov, K. S. Plokhikh, N. Yu. Lotosh & A. A. Selishcheva

  3. Moscow State University, Moscow, Russia

    A. A. Selishcheva

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  1. N. S. Marchenkova
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  2. K. E. Barkar
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  3. E. A. Kulikov
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  5. N. Yu. Lotosh
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  6. A. A. Selishcheva
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Correspondence to E. A. Kulikov.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

All studies were conducted in accordance with the principles of biomedical ethics as outlined in the 1964 Declaration of Helsinki and its later amendments. They were also approved by the Local Ethics Committee on Biomedical Research of the National Research Center “Kurchatov Institute,” no. NG-1/01.13 dated November 3, 2021.

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Marchenkova, N.S., Barkar, K.E., Kulikov, E.A. et al. Liposomes Containing Esters of the Natural Antioxidant Astaxanthin Modified with Pluronic F68 or DSPE-PEG 2000. Nanotechnol Russia 18 (Suppl 2), S240–S249 (2023). https://doi.org/10.1134/S2635167623601511

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  • DOI: https://doi.org/10.1134/S2635167623601511

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