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Leukocyte telomere length as an aging marker and risk factor for human age-related diseases

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Abstract

Leukocyte telomere length (LTL) associations with age, sex, and risk factors for age-related diseases have been studied in Russian people of preretirement and retirement age. This parameter has been determined by quantitative real-time PCR in 398 men (56.3 ± 7.2 years) and 365 women (56.6 ± 7.1 years) selected from a population sample of 45 to 69-year-old residents of the Oktyabrskii and Kirovskii raions of Novosibirsk (9400 people). The sample was formed in the course of the international project HAPIEE. Telomere length has been found to correlate with age (r =–0.159, p < 0.001) and the waist: hip ratio (WHR) (r =–0.107, p = 0.003). The average LTL in women is significantly greater than in men, p = 0.031. In men, LTL correlates with body mass (r = 0.140, p = 0.005) and waist size (r = 0.111, p = 0.027). In women, there are inverse correlations of LTL with waist size (r =–0.127, p = 0.015) and WHR (r =–0.141, p = 0.007). Leukocyte telomere length inversely correlates with the amount smoked (r =–0.121, р = 0.024). It directly correlates with age, smoking, and a variety of phenotypical traits. In men with family histories of malignancies, LTL is greater than in men without such histories.

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References

  1. Smirnova, T.Yu., Runov, A.L., Vonsky, M.S., Spivak, D.L., Zakharchuk, A.G., Mikhelson, V.M., and Spivak, I.M., Telomere length in a population of long-lived people of the northwestern region of Russia, Cell Tissue Biol., 2012, vol. 6, no. 5, pp. 465–471.

    Article  Google Scholar 

  2. Smith, C.L., Kalco, S.R., and Cantor, C., Pulsed field gel electrophoresis and the technology of large DNA molecules, in Genome Analysis: A Practical Approach, Davies, K.E., Ed., Oxford: IRL, 1988, pp. 41–72.

    Google Scholar 

  3. Aviv, A., Valdes, A.M., and Spector, T.D., Human telomere biology: pitfalls of moving from the laboratory to epidemiology, Int. J. Epidemiol., 2006, vol. 35, pp. 1424–1429.

    Article  PubMed  Google Scholar 

  4. Baerlocher, G.M., Vulto, I., De Jong, G., and Lansdorp, P.M., Flow cytometry and FISH to measure the average length of telomeres (flow FISH), Nat. Protoc., 2006, vol. 5, no. 1, pp. 2365–2376.

    Article  Google Scholar 

  5. Barrett, E.L. and Richardson, D.S., Sex differences in telomeres and lifespan, Aging Cell, 2011, vol. 10, no. 6, pp. 913–921.

    Article  CAS  PubMed  Google Scholar 

  6. Bendix, L., Thinggaard, M., Fenger, M., et al., Longitudinal changes in leukocyte telomere length and mortality in humans, J. Gerontol., Ser. A, 2014, vol. 69, no. 2, pp. 231–239.

    Article  CAS  Google Scholar 

  7. Benetos, A., Kark, J.D., Susser, E., et al., Tracking and fixed ranking of leukocyte telomere length across the adult life course, Aging Cell, 2013, vol. 12, no. 4, pp. 615–621.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Bischoff, C., Petersen, H.C., Graakjaer, J., et al., Noassociation between telomere length and survival among the elderly and oldest old, Epidemiology, 2006, vol. 17, no. 2, pp. 190–194.

    Article  PubMed  Google Scholar 

  9. Carty, C.L., Kooperberg, C., Liu, J., et al., Leukocyte telomere length and risks of incident coronary heart disease and mortality in a racially diverse population of postmenopausal women, Arterioscler., Thromb., Vasc. Biol., 2015, vol. 35, no. 10, pp. 2225–2231.

    Article  CAS  Google Scholar 

  10. Cawthon, R.M., Telomere measurement by quantitative PCR, Nucleic Acids Res., 2002, vol. 30, no. 10, p. e47.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Cawthon, R.M., Smith, K.R., O’Brien, E., et al., Association between telomere length in blood and mortality in people aged 60 years or older, Lancet, 2003, vol. 361, pp. 393–395.

    Article  CAS  PubMed  Google Scholar 

  12. Deelen, J., Beekman, M., Codd, V., et al., Leukocyte telomere length associates with prospective mortality independent of immune-related parameters and known genetic markers, Int. J. Epidemiol., 2014, vol. 43, no. 3, pp. 878–886.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Fitzpatrick, A.L., Kronmal, R.A., Gardner, J.P., et al., Leukocyte telomere length and cardiovascular disease in the cardiovascular health study, Am. J. Epidemiol., 2007, vol. 165, no. 1, pp. 14–21.

    Article  PubMed  Google Scholar 

  14. Gardner, M., Bann, D., Wiley, L., et al., Gender and telomere length: systematic review and meta-analysis, Exp. Gerontol., 2014, vol. 51, pp. 15–27.

    Article  CAS  PubMed  Google Scholar 

  15. Harris, S.E., Deary, I.J., MacIntyre, A., et al., The association between telomere length, physical health, cognitive ageing, and mortality in nondemented older people, Neurosci. Lett., 2006, vol. 406, no. 3, pp. 260–264.

    Article  CAS  PubMed  Google Scholar 

  16. Hills, M., Lücke, K., Chavez, E.A., et al., Probing the mitotic history and developmental stage of hematopoietic cells using single telomere length analysis (STELA), Blood, 2009, vol. 113, no. 23, pp. 5765–5775.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. http://www.ucl.ac.uk/easteurope/hapiee.html.

  18. Hunt, S.C., Chen, W., Gardner, J.P., et al., Leukocyte telomeres are longer in African Americans than in whites: the National Heart, Lung, and Blood Institute Family Heart Study and the Bogalusa Heart Study, Aging Cell, 2008, vol. 7, no. 4, pp. 451–458.

    CAS  PubMed  Google Scholar 

  19. Kimura, M., Hjelmborg, J.V., Gardner, J.P., et al., Telomere length and mortality: a study of leukocytes in elderly Danish twins, Am. J. Epidemiol., 2008, vol. 167, no. 7, pp. 799–806.

    Article  PubMed  PubMed Central  Google Scholar 

  20. Lan, Q., Cawthon, R., Gao, Y., et al., Longer telomere length in peripheral white blood cells is associated with risk of lung cancer and the rs2736100 (CLPTM1LTERT) polymorphism in a prospective cohort study among women in China, PLoS One, 2013, vol. 8, no. 3, p. e59230.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Lee, J.H., Cheng, R., Honig, L.S., et al., Genome wide association and linkage analyses identified three loci- 4q25, 17q23.2, and 10q11.21-associated with variation in leukocyte telomere length: the Long Life Family Study, Front. Genet., 2014, vol. 4, p. 310.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Lynch, S.M., Major, J.M., Cawthon, R., et al., A prospective analysis of telomere length and pancreatic cancer in the alphatocopherol betacarotene cancer (ATBC) prevention study, Int. J. Cancer, 2013, vol. 133, no. 11, pp. 2672–2680.

    CAS  PubMed  Google Scholar 

  23. Martin-Ruiz, C.M., Gussekloo, J., van Heemst, D., et al., Telomere length in white blood cells is not associated with morbidity or mortality in the oldest old: a population-based study, Aging Cell, 2005, vol. 4, no. 6, pp. 287–290.

    Article  CAS  PubMed  Google Scholar 

  24. Müezzinler, A., Mons, U., Dieffenbach, A.K., et al., Smoking habits and leukocyte telomere length dynamics among older adults: results from the ESTHER cohort, Exp. Gerontol., 2015, vol. 70, pp. 18–25.

    Article  PubMed  Google Scholar 

  25. Newman, A.B. and Murabito, J.M., The epidemiology of longevity and exceptional survival, Epidemiol. Rev., 2013, vol. 35, pp. 181–197.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Nordfjall, K., Eliasson, M., Stegmayr, B., et al., Increased abdominal obesity, adverse psychosocial factors and shorter telomere length in subjects reporting early ageing; the MONICA Northern Sweden Study, Scand. J. Publ. Health, 2008, vol. 36, no. 7, pp. 744–752.

    CAS  Google Scholar 

  27. O’Callaghan, N.J. and Fenech, M., A quantitative PCR method for measuring absolute telomere length, Biol. Proced. Online, 2011, vol. 13, p. 3.

    Article  PubMed  PubMed Central  Google Scholar 

  28. Peasey, A., Bobak, M., Kubinova, R., et al., Determinants of cardiovascular disease and other non-communicable diseases in Central and Eastern Europe: rationale and design of the HAPIEE study, BMC Publ. Health, 2006, vol. 6, p. 255.

    Article  Google Scholar 

  29. Reste, J., Zvigule, G., Zvagule, T., et al., Telomere length in Chernobyl accident recovery workers in the late period after the disaster, J. Radiat. Res., 2014, vol. 55, no. 6, pp. 1089–100.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Révész, D., Milaneschi, Y., Verhoeven, J.E., et al., Longitudinal associations between metabolic syndrome components and telomere shortening, J. Clin. Endocrinol. Metab., 2015, vol. 100, no. 8, pp. 3050–3059.

    Article  PubMed  Google Scholar 

  31. Roux, A.V.D., Ranjit, N., Jenny, N.S., et al., Race/ethnicity and telomere length in the multi-ethnic study of atherosclerosis, Aging Cell, 2009, vol. 8, no. 3, pp. 251–257.

    Article  Google Scholar 

  32. Sebastiani, P., Riva, A., Montano, M., et al., Whole genome sequences of a male and female supercentenarian, ages greater than 114 years, Front. Genet., 2012, no. 2, p. 90.

    Article  PubMed  PubMed Central  Google Scholar 

  33. Von Zglinicki, T. and Martin-Ruiz, C.M., Telomeres as biomarkers for ageing and age-related diseases, Curr. Mol. Med., 2005, vol. 5, no. 2, pp. 197–203.

    Article  Google Scholar 

  34. Willeit, P., Willeit, J., Mayr, A., et al., Telomere length and risk of incident cancer and cancer mortality, JAMA, J. Am. Med. Assoc., 2010, vol. 304, no. 1, pp. 69–75.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Internal and Preventive Medicine, Novosibirsk, 630089, Russia

    V. N. Maximov, S. K. Malyutina, P. S. Orlov, D. E. Ivanoschuk, E. N. Voropaeva & M. I. Voevoda

  2. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia

    V. N. Maximov, P. S. Orlov, D. E. Ivanoschuk & M. I. Voevoda

  3. University College London, London, WC1E6BT, UK

    M. Bobak

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  1. V. N. Maximov
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  2. S. K. Malyutina
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Correspondence to V. N. Maximov.

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Original Russian Text © V.N. Maximov, S.K. Malyutina, P.S. Orlov, D.E. Ivanoschuk, E.N. Voropaeva, M. Bobak, M.I. Voevoda, 2016, published in Uspekhi Gerontologii, 2016, Vol. 29, No. 5, pp. 702–708.

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Maximov, V.N., Malyutina, S.K., Orlov, P.S. et al. Leukocyte telomere length as an aging marker and risk factor for human age-related diseases. Adv Gerontol 7, 101–106 (2017). https://doi.org/10.1134/S2079057017020102

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