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Gene-Activated Hydrogels Based on Sodium Alginate for Reparative Myogenesis of Skeletal Muscle

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Inorganic Materials: Applied Research Aims and scope

Abstract

Two variants of gene-activated hydrogels based on sodium alginate containing plasmid DNA with the gene of vascular endothelial growth factor (VEGF-A) are developed. The former represented alginate hydrogel without additional components; the latter contained up to 25 wt % of octacalcium phosphate (OCP) micrograins. Alginate-based hydrogels without OCP are characterized by a honeycomb structure with the pore size of 50–200 μm and mechanical compression strength of 0.2 MPa. Addition of OCP micrograins results in filling of the polymer framework by them and an increase in the compression strength to 0.57 MPa at 16.7 wt % of OCP with a decrease to 0.49 MPa with an increase in the OCP content to 20 wt %. Both variants of gene-activated hydrogels induce reparative myogenesis in the central zone of muscle defect; a larger number of MyoG+ cells and newly formed MyH7B+ muscle fibers are identified as compared to analogous hydrogels without plasmid DNA after two weeks after surgery (p < 0.05).

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ACKNOWLEDGMENTS

We are grateful to A.A. Pulin and I.I. Eremin for participation in the study.

Funding

This work was supported by the Russian Science Foundation (agreement no. 18-75-10085 on August 8, 2018).

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Authors and Affiliations

  1. Research Institute of General Pathology and Pathophysiology, 125315, Moscow, Russia

    I. Ya. Bozo & M. I. Yasinovsky

  2. Burnazyan Federal Medical Biophysical Center, Federal Medical and Biological Agency of Russia, 123098, Moscow, Russia

    I. Ya. Bozo

  3. OOO Gistograft, 121205, Moscow, Russia

    I. Ya. Bozo, E. V. Presnyakov, V. O. Trofimov & R. V. Deev

  4. Kazan (Volga Region) Federal University, 420008, Kazan, Russia

    M. O. Mavlikeev, A. R. Babkova, A. A. Titova & A. I. Bilyalov

  5. Kazan State Medical University, 420012, Kazan, Russia

    F. A. Indeykin

  6. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, 119334, Moscow, Russia

    O. V. Baranov & V. S. Komlev

  7. PAO Human Stem Cells Institute, 117312, Moscow, Russia

    A. A. Isaev

Authors
  1. I. Ya. Bozo
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  2. M. O. Mavlikeev
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  3. E. V. Presnyakov
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  4. M. I. Yasinovsky
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  5. V. O. Trofimov
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  6. F. A. Indeykin
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  7. A. R. Babkova
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  8. A. A. Titova
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  9. A. I. Bilyalov
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  10. O. V. Baranov
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  11. V. S. Komlev
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  12. A. A. Isaev
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  13. R. V. Deev
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Corresponding author

Correspondence to V. S. Komlev.

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Translated by A. Muravev

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Bozo, I.Y., Mavlikeev, M.O., Presnyakov, E.V. et al. Gene-Activated Hydrogels Based on Sodium Alginate for Reparative Myogenesis of Skeletal Muscle. Inorg. Mater. Appl. Res. 12, 1026–1032 (2021). https://doi.org/10.1134/S2075113321040092

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  • DOI: https://doi.org/10.1134/S2075113321040092

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