Abstract
The composition and properties of a wide variety of domestic and foreign enzyme preparations (EP) used as additives to feeds of farm animals and poultry are analyzed. Content of the main active enzymes – endoglucanases (beta-glucanases), cellobiohydrolases, and xylanases performing biocatalytic destruction of non-starch polysaccharides, which are anti-nutritional factors in feeds causing their incomplete digestion, is determined. It is shown that based on the component composition data and the level of different types of activity the studied enzyme preparations can be classified into three groups: a) with high xylanase and low cellulase (endoglucanase and cellobiohydrolase) content, b) with high cellulase and low xylanase content, c) containing cellobiohydrolases, endoglucanases and xylanases at a different ratio, but without significant prevalence of any of these enzymes. The ability of EP to reduce the viscosity of water-soluble non-starch polysaccharides – xylans and beta-glucans – has been studied. Among the enzyme preparations that have xylanase in their composition and belong to the groups b) and c) a number of preparations have been identified (Econase XT 25, Agroxyl Plus, Agroxyl Premium, Rovabio Max AP, Sunzyme), which reduced the viscosity of aqueous extract of rye containing xylans most effectively when used at the same dosage with regards to xylanase activity. It was shown that xylanase from precisely these EP was not inhibited by the protein inhibitors of rye. The viscosity of water-soluble beta-glucans of barley was most effectively reduced by the EP Xybeten CELL, Cellulase, Agroxyl, Agrocel, Axtra XB 201, Rovabio Max AP, and Vilzim used at the same dosage with regards to beta-glucanase activity. For all the studied EP, no inhibitory effect of the barley extract on beta-glucanase activity was found.
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ACKNOWLEDGEMENTS
This work was financially supported by the Ministry of Education and Science of the Russian Federation, Federal Targeted Program “Investigation and development of the priority direction of the progress of the scientific-technological complex of Russia for 2014–2020” (Identification no. PNIER RFMEFI60716X0159). Scientific equipment of the Center for Collective Use “Industrial Biotechnology” and the Center for Collective Use “Bioengineering” in the Federal Research Center, Russian Academy of Sciences, was used in this work.
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Korotkova, O.G., Rubtsova, E.A., Shashkov, I.A. et al. Comparative Analysis of the Composition and Properties of Fodder Enzyme Preparations. Catal. Ind. 10, 368–374 (2018). https://doi.org/10.1134/S2070050418040098
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DOI: https://doi.org/10.1134/S2070050418040098