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Prosthecate Bacterium Asticcacaulis sp. VKM B-2400 from Ochreous Creek Deposits and Its Growth upon a High Iron Content

  • WATER MICROBIOLOGY
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Abstract

This paper reports the isolation and identification of the stalked prosthecate bacterium Asticcacaulis sp. VKM B-2400 culture from the ochreous deposits of a creek with predominating iron-oxidizing bacteria. Molecular genetic analysis has demonstrated that, according to the nucleotide sequence of the 1368 bp 16S rRNA gene fragment, the isolated strain is most closely related (99% identity) to the A. taihuensis species previously described based on a single strain. A description of the Asticcacaulis sp. strain VKM B-2400 is provided. It has been demonstrated that the isolated bacteria, the inhabitants of ochreous deposits, neither convert nor accumulate Fe3+ compounds and transform their metabolism at high dissolved iron concentrations (0.5 mM). The indicators of the change in metabolism were biomass peptide composition and the accumulation of storage organic compounds in the bacterial cells. The data obtained in the present article is the first report of the effects of high concentrations of dissolved iron (Fe3+) on the metabolism of bacteria not belonging to iron-oxidizing bacteria.

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Funding

This work was supported by the State Task of the Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (project no. 007-00459-18-00).

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    G. V. Khokhlova, V. N. Akimov, K. S. Laurinavichus, T. N. Abashina, N. E. Suzina & M. B. Vainshtein

  2. Tula State University, 300600, Tula, Russia

    K. A. Komarova

  3. Pushchino State Institute of Natural Sciences, 142290, Pushchino, Moscow oblast, Russia

    T. N. Abashina

  4. Institute of Biological and Physico-Chemical Problems of Soil Sciences, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    V. E. Ostroumov

Authors
  1. G. V. Khokhlova
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  2. K. A. Komarova
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  3. V. N. Akimov
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  4. K. S. Laurinavichus
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  5. T. N. Abashina
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  6. N. E. Suzina
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  7. V. E. Ostroumov
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  8. M. B. Vainshtein
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Corresponding author

Correspondence to G. V. Khokhlova.

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No experimentation involving animals was part of the present work. The authors declare that they have no conflict of interest.

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Translated by E. Martynova

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Khokhlova, G.V., Komarova, K.A., Akimov, V.N. et al. Prosthecate Bacterium Asticcacaulis sp. VKM B-2400 from Ochreous Creek Deposits and Its Growth upon a High Iron Content. Inland Water Biol 12 (Suppl 1), 31–36 (2019). https://doi.org/10.1134/S1995082919050110

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  • DOI: https://doi.org/10.1134/S1995082919050110

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