Abstract
A novel selenium nanobiocomposite consisting of X-ray amorphous nanoparticles of red elemental selenium (average size ∼67 nm) uniformly encapsulated into arabinogalactan macromolecules has been synthesized. The effect of this nanobiocomposite and its precursors (pure arabinogalactan and selenium dioxide) on the viability of a phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus (Spieck et Kotth.) Skapt et Burkh (C. michiganensis) and potato plants has been investigated in vitro. The bactericidal effects of free selenium dioxide and the nanocomposite obtained from the former (normalized selenium concentration 0.000625%) have been demonstrated. The targeted metabolic (trophic) delivery of antimicrobial selenium nanoparticles to the bacterial cells by the arabinogalactan matrix incorporating the particles was detected in the latter case. The treatment of potato plants with selenium dioxide was found to have a negative effect on peroxidase activity and plant growth. On the contrary, treating the plants with the selenium nanocomposite had no negative effects. Thus, the nanocomposite of elemental selenium and arabinogalactan produced in the present work can be considered a new antimicrobial agent for plant sanitation with minimal side effects.
Similar content being viewed by others
References
R. Eichenlaub and K. H. Gartemann, “The clavibacter michiganensis subspecies: molecular investigation of gram-positive bacterial plant pathogens,” Annu. Rev. Phytopathol. 49, 445–464 (2011).
“Clavibacter michiganensis subsp. Sepedonicus,” Data Sheets on Quarantine Pests. Prepared by CABI and EPPO for the EU under Contract No. 90/399003 (2005). http://www.eppo.int/QUARANTINE/bacteria/Clavibactersepedonicus/CORBSE.ds.pdf
B. V. Anisimov, G. L. Belov, Yu. A. Varitsev, S. N. Elanskii, G. K. Zhuromskii, S. K. Zavriev, V. N. Zeiruk, V. G. Ivanyuk, M. A. Kuznetsova, M. P. Plyakhnevich, K. A. Pshechenkov, E. A. Simakov, N. P. Sklyarova, Z. Stashevski, A. I. Uskov, and I. M. Yashina, The Way to Protect Potato from Diseases, Invaders and Weeds (Kartofelevod, Moscow, 2009) [in Russian].
G. A. Secor, L. De Buhr, and N. C. Gudmestad, “Susceptibility of corynebacterium sepedonicum to disinfectants in vitro,” Plant Disease 72(7), 585–588 (1988).
A. Travan, E. Marsich, I. Donati, and S. Paoletti, “Silver nanocomposites and their biomedical applications,” in Nanotechnologies for the Life Sciences (John Wiley and Sons, 2012), pp. 81–137.
C. Nicola and R. Mahendra, Nano-Antimicrobials: Progress and Prospects (Springer, Berlin, New York, 2012).
S. Eckhardt, P. S. Brunetto, J. Gagnon, M. Priebe, B. Giese, and K. M. Fromm, “Nanobio silver: its interactions with peptides and bacteria, and its uses in medicine,” Chem. Rev. 113(7), 4708–4754 (2013).
S. Chernousova and M. Epple, “Silver as antibacterial agent: ion, nanoparticle, and metal,” Angew. Chem. 52(6), 1636–1653 (2013).
B. A. Trofimov, B. G. Sukhov, G. P. Aleksandrova, S. A. Medvedeva, L. A. Grishchenko, A. G. Mal’kina, L. P. Feoktistova, A. N. Sapozhnikov, V. I. Dubrovina, E. F. Martynovich, V. V. Tirskii, and A. L. Semenov, “Nanocomposites with magnetic, optical, catalytic and biological properties based on arabinogalactan,” Dokl. Akad. Nauk 393(5), 634–635 (2003).
G. P. Aleksandrova, L. A. Grishchenko, T. V. Fadeeva, S. A. Medvedeva, B. G. Sukhov, and B. A. Trofimov, RF Patent No. 2278669 (2006).
G. P. Aleksandrova, L. A. Grishchenko, T. V. Fadeeva, B. G. Sukhov, and B. A. Trofimov, “Formation features for silver and golden nanobiocomposites with antimicrobial activity,” Nanotekhnika, No. 23, 34–42 (2010).
M. V. Lesnichaya, G. P. Aleksandrova, L. P. Feoktistova, A. N. Sapozhnikov, T. V. Fadeeva, B. G. Sukhov, and B. A. Trofimov, “Silver containing nanocomposites based on galactomannan and carageenan: synthesis, structure, antimicrobial properties,” Russ. Chem. Bull. Int. Ed., No. 12, 2323–2328 (2010).
T. V. Ganenko, Ya. A. Kostyro, B. G. Sukhov, B. A. Trofimov, T. V. Fadeeva, S. A. Vereshchagina, and L. B. Koryakina, RF Patent No. 2462254 (2012).
Ya. A. Kostyro, K. V. Alekseev, E. N. Petrova, E. N. Gumennikova, T. V. Romanko, V. G. Romanko, S. A. Lepekhova, I. A. Shurygina, L. B. Koryakina, T. V. Fadeeva, S. A. Vereshchagina, E. V. Koval’, M. G. Shurygin, V. A. Babkin, T. V. Ganenko, L. A. Grishchenko, L. A. Ostroukhova, B. G. Sukhov, and B. A. Trofimov, RF Patent No. 2513186 (2014).
T. V. Fadeeva, I. A. Shurygina, B. G. Sukhov, M. K. Rai, M. G. Shurygin, V. A. Umanets, M. V. Lesnichaya, T. V. Kon’kova, and D. M. Shurygin, “Relationship between the structures and antimicrobial activities of argentic nanocomposites,” Bull. Russ. Acad. Sci. Phys. 79(2), 273–275 (2015).
I. A. Shurygina, B. G. Sukhov, T. V. Fadeeva, V. A. Umanets, M. G. Shurygin, T. V. Ganenko, Ya. A. Kostyro, E. G. Grigoriev, and B. A. Trofimov, “Bactericidal action of Ag(0)-antithrombotic sulfated arabinogalactan nanocomposite: coevolution of initial nanocomposite and living microbial cell to a novel nonliving nanocomposite,” Nanomed.: Nanotechnol., Biol. Med. 7(6), 827–833 (2011).
I. A. Graskova, G. B. Borovskii, and B. G. Sukhov, “Bactericide impact of polymer-stabilized multi-functional nano-composites,” J. Stress Physiol. Biochem. 8(3), S33 (2012).
A. I. Perfil’eva, A. V. Papkina, G. B. Borovskii, B. G. Sukhov, I. A. Graskova, and B. A. Trofimov, “Selenium nanocomposite effect onto Clavibacter michiganensis subsp. Sepedonicus as a function of hydrocarbons content in medium,” Izv. Vyssh. Uchebn. Zaved. Prikl. Khim. Biotekhnol. 5(2), 52–56 (2013).
B. G. Sukhov, N. N. Pogodaeva, S. V. Kuznetsov, Yu. N. Kupriyanovich, G. V. Yurinova, D. S. Selivanova, A. A. Pristavka, Yu. P. Dzhioev, S. M. Popkova, E. B. Rakova, P. A. Medvedeva, and B. A. Trofimov, “Prebiotic effect of native noncovalent arabinogalactan—flavonoid conjugates on bifidobacteria,” Russ. Chem. Bull. Int. Ed. 63(9), 1–6 (2014).
A. R. Shahverdi, A. Fakhimi, G. Mosavat, P. Jafari-Fesharaki, S. Rezaie, and S. M. Rezayat, “Antifungal activity of biogenic selenium nanoparticles,” World Appl. Sci. J. 10(8), 918–922 (2010).
P. Tran and T. Webster, “Selenium nanoparticles inhibit staphylococcus aureus growth,” Int. J. Nanomed., No. 6, 1553–1558 (2011).
T. Schneider, A. Baldauf, L. A. Ba, V. Jamier, K. Khairan, M. Sarakbi, N. Reum, M. Schneider, A. Reseler, K. Becker, T. Burkholz, P. G. Winyard, M. Kelkel, M. Diederich, and C. Jacob, “Selective antimicrobial activity associated with sulfur nanoparticles,” J. Biomed. Nanotechnol. 7, 1–11 (2011).
Q. Wang and T. J. Webster, “Nanostructured selenium for preventing biofilm formation on polycarbonate medical devices,” J. Biomed. Mater. Res. A 100(12), 3205–3210 (2012).
Q. Wang and T. J. Webster, “Short communication: inhibiting biofilm formation on paper towels through the use of selenium nanoparticles coatings,” Int. J. Nanomed. 8, 407–411 (2013).
P. A. Tran and T. J. Webster, “Antimicrobial selenium nanoparticle coatings on polymeric medical devices,” Nanotecnology 24(15), 1551 (2013).
A. I. Ermakov, V. V. Arasimovich, N. P. Yarosh, et al., Biochemical Research Methods for Plants (Agropromizdat, Leningrad, 1987) [in Russian].
E. Kh. Padu, “Properties of peroxydase and phenylalanyl-ammonia-lyase under formation and lignification of wheat cell walls,” Fiziol. Rastenii 42(3), 408–415 (1995).
D. H. Bergey, J. G. Holt, and P. H. A. Sneath, Bergey’s Manual of Systematic Bacteriology, Ed. by P. H. A. Sneath (Williams & Wilkins, 1986).
V. A. Andreeva, Peroxydase. Involvement in Plant Defense Mechanism (Nauka, Moscow, 1988).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.V. Papkina, A.I. Perfileva, M.A. Zhivet’yev, G.B. Borovskii, I.A. Graskova, I.V. Klimenkov, M.V. Lesnichaya, B.G. Sukhov, B.A. Trofimov, 2015, published in Rossiiskie Nanotekhnologii, 2015, Vol. 10, Nos. 5–6.
Rights and permissions
About this article
Cite this article
Papkina, A.V., Perfileva, A.I., Zhivet’yev, M.A. et al. Complex effects of selenium-arabinogalactan nanocomposite on both phytopathogen Clavibacter michiganensis subsp. sepedonicus and potato plants. Nanotechnol Russia 10, 484–491 (2015). https://doi.org/10.1134/S1995078015030131
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1995078015030131