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Evaluation of the Polyphenol Composition and Acetylcholinesterase Inhibitory Activity of Ginkgo Biloba Leaf Extracts Produced in Subcritical Water

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Abstract

Ginkgo biloba (GB) leaf extracts are obtained in subcritical water (SBW) in the temperature range 100–200°C and studied for the first time in order to evaluate their potential in the prevention and treatment of neurodegenerative diseases, such as Alzheimer’s disease (AD). The relationship between the acetylcholinesterase (AChE) inhibitory activity of the extracts, expressed as IC50 values (the concentration of extract at which the AChE activity is inhibited by 50%) determined from the dose–effect curves, and the contents of polyphenols and flavonoids there is determined. The content of polyphenols and flavonoids in the extracts and their AChE inhibitory activity are shown to depend on the extraction method and conditions. The maximum anti-AChE activity is exhibited by the SBW extract obtained at 220°C (IC50 = 0.74 mg mL–1), while that of the conventional water–ethanol extract is IC50 = 3.57 mg mL–1. The anti-AChE activity of the GB leaf extracts is shown to be correlated to the total content of polyphenols and flavonoids.

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Funding

This research was financially supported by the Ministry of Science and Higher Education of the Russian Federation (State assignment in the field of scientific activity, project no. 0852-2020-0031).

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Authors and Affiliations

  1. Institute of Physical and Organic Chemistry, Southern Federal University, Rostov-on-Don, Russia

    S. S. Khizrieva, S. N. Borisenko, E. V. Maksimenko, G. V. Zharkova, N. I. Borisenko & V. I. Minkin

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  1. S. S. Khizrieva
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  2. S. N. Borisenko
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  3. E. V. Maksimenko
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  4. G. V. Zharkova
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  5. N. I. Borisenko
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  6. V. I. Minkin
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Correspondence to N. I. Borisenko.

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Translated by K. Utegenov

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Khizrieva, S.S., Borisenko, S.N., Maksimenko, E.V. et al. Evaluation of the Polyphenol Composition and Acetylcholinesterase Inhibitory Activity of Ginkgo Biloba Leaf Extracts Produced in Subcritical Water. Russ. J. Phys. Chem. B 16, 1294–1300 (2022). https://doi.org/10.1134/S1990793122070090

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  • DOI: https://doi.org/10.1134/S1990793122070090

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